Cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. To improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. The first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. Morphol...

Glutathione S-transferase (GST) activity pattern was determined in tolerant (cv. Sumai#3) and susceptible (cv. Falat) wheat heads inoculated with Fusarium graminearum, the causal agent of head scab disease (FHB), during various developmental stages. GST specific activity exhibited a transient pattern in Sumai#3 reaching a maximum level at the milk stage and declining thereafter. GST level in Su...

One hundred fifty-two institutionalized persons with late-stage dementia were rated with a geriatric developmental screening scale that notes cognitive, language, sensorimotor, and self-care abilities. The results showed that the loss of functional abilities in late-stage dementias seems to occur in reverse order of their development in childhood. Developmental congruence was noted within these...

In animals that do not regulate their body temperature by the production of heat, the proportion of the total developmental time spent in a particular developmental stage does not change with temperature. In the quasi-linear region of the relationship between developmental rate and temperature, all of the developmental stages appear to have the same species-specific lower developmental threshol...

Molecular mechanisms determining methylation patterns in eukaryotic genomes still remain unresolved. We have characterized, in Ascobolus, a gene for de novo methylation. This novel eukaryotic gene, masc1, encodes a protein that has all motifs of the catalytic domain of eukaryotic C5-DNA-methyltransferases but is unique in that it lacks a regulatory N-terminal domain. The disruption of masc1 has...

Selection of fungal strains with high virulence against the developmental stages of Bemisia tabaci was performed using internal transcribed spacer regions. The growth rate of hyphae was measured and bioassay of each developmental stage of B. tabaci was conducted for seven days. All of the fungal strains tested were identified as Lecanicillium spp., with strain 4078 showing the fastest mycelium ...

The life history of Bemisia argentifolii Bellows & Perring (Homoptera: Aleyrodidae) on tomato (Lycopersicum spp.) was studied based on the age-stage, two-sex life table at 15, 20, 25, 28, 30, and 35 degrees C. The intrinsic rate of increase (r) at these temperatures is -0.0176, 0.0667, 0.1469, 0.1611, 0.1745, and 0.0989 d(-1), respectively. The relationship among the gross reproductive rate (GR...

Embryonic gene expression patterns are an indispensable part of modern developmental biology. Currently, investigators must visually inspect numerous images containing embryonic expression patterns to identify spatially similar patterns for inferring potential genetic interactions. The lack of a computational approach to identify pattern similarities is an impediment to advancement in developme...

The early preclinical development of an antiviral agent is accomplished essentially in two stages. The first stage consists of gathering data to estimate the potential therapeutic index of the agent. This process includes testing for antiviral activity and for cytotoxicity in vitro and performing preliminary pharmacokinetic and toxicology studies in vivo. The second stage consists of carrying o...

Twelve accessions of tronchuda cabbage landraces were tested for their ability to produce embryos through microspore culture in NLN-13 medium. A sample from all the isolations was stained with DAPI for determination of microspore developmental stage. The relationship between the microspore developmental stage and the production of microspore-derived embryos was evaluated. Embryos were obtained ...