Abstract Study question Do the in vitro motility characteristics of sperm collected using a microfluidic device (ZyMot) differ from those by density gradient centrifugation (DGC)? Summary answer ZyMot-collected had longer-lasting than DGC, but head beat-cross frequency was inferior due to absence washing vitro. What is known already ZyMot (DxNow, Inc., USA) can sort highly motile with low react...

The memory management of distributed objects, when done manually, is an error-prone task. It leads to memory leaks and dangling references causing applications to fail. Avoiding such errors requires automatic memory management, named distributed garbage collection (DGC). Current DGC solutions are either not safe, not complete or not portable to widely used platforms such as .NET. As a matter of...

Dp116 is a non-muscle isoform of dystrophin that assembles the dystrophin-glycoprotein complex (DGC), but lacks actin-binding domains. To examine the functional role of the DGC, we expressed the Dp116 transgene in mice lacking both dystrophin and utrophin (mdx:utrn(-/-)). Unexpectedly, expression of Dp116 prevented the most severe aspects of the mdx:utrn(-/-) phenotype. Dp116:mdx:utrn(-/-) tran...

The relationship of the total arsenic content of a soil and its bioaccumulation by earthworms (Lumbricus rubellus and Dendrodrilus rubidus) to the arsenic fraction bioaccessible to humans, measured using an in vitro physiologically-based extraction test (PBET), was investigated. Soil and earthworm samples were collected at 24 sites at the former arsenic mine at the Devon Great Consols (DGC) in ...

This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influence of sperm preparation on sperm quality after incubation. Progressive motili...

The memory management of distributed objects, when done manually, is an error-prone task. It leads to memory leaks and dangling references, causing applications to fail. Avoiding such errors requires automatic memory management, called distributed garbage collection (DGC). Current DGC solutions are either not safe, not complete or not portable to widely used platforms such as .NET. As a matter ...

Neurogenesis in the hippocampal dentate gyrus persists throughout life and is increased by seizures. The dentate granule cell (DGC) layer is often abnormal in human and experimental temporal lobe epilepsy, with dispersion of the layer and the appearance of ectopic granule neurons in the hilus. We tested the hypothesis that these abnormalities result from aberrant DGC neurogenesis after seizure-...

A major role for Müller cells in the retina is to buffer changes in the extracellular K+ concentration ([K+]o) resulting from light-evoked neuronal activity. The primary K+ conductance in Müller cells is the inwardly rectifying K+ channel Kir4.1. Since this channel is constitutively active, K+ can enter or exit Müller cells depending on the state of the [K+]o. This process of [K+]o buffering by...

The termination of discontinuous gas exchange cycles (DGCs) in severely dehydrated insects casts doubt on the generality of the hygric hypothesis, which posits that DGCs evolved as a water conservation mechanism. We followed DGC characteristics in the two density-dependent phases of the desert locust Schistocerca gregaria throughout exposure to an experimental treatment of combined dehydration ...

Without the SNF-6 acetylcholine transporter (bottom) mutant muscles fall apart. K im /M ac m ill an they are mutant for various members of the dystrophinglycoprotein complex (DGC), which links intracellular actin to extracellular laminin. But now, Hongkyun Kim, Steven McIntire (University of California, San Francisco, CA), and colleagues suggest that loss of the DGC results in loss of an associ...