Different subtypes of GABAA (gamma-aminobutyric acid A) receptors, through their specific regional and cellular localization, are involved in the manifestation various functions, both at central peripheral levels. We hypothesized that non-neuronal receptors expressed on blood vessels, which positive allosteric modulators exhibit vasodilatory effects. This study two parts: one to determine prese...

This study aimed to evaluate the sleep modulating properties of Strychnos spinosa and innocua fruits in Swiss albino mice. The depressant effect extracts on central nervous system was studied using a diazepam-induced model mice were divided into four groups six each. test received at doses 250, 500, 1000 mg/kg body weight intraperitoneally. In contrast, control group distilled water 10 ml/kg. D...

background anxiety is a physiological state characterized by cognitive, somatic, emotional, and behavioral components. there is some evidence in traditional medicine for the effectiveness of lettuce (lactuca sativa) in the treatment of anxiety in humans. objectives the present study investigated the effects of a hydro-alcoholic extract of lettuce on rat behavior in the elevated plus-maze (epm) ...

We report the case of a five-year-old girl, presenting with difficult-to-treat, symptomatic focal epilepsy, who developed status gelasticus following the introduction of levetiracetam as add-on treatment to oxcarbazepine and diazepam. Gelastic seizures were documented by video-EEG and were responsive to i.v. administration of diazepam. A possible causative role of levetiracetam is suggested. Sp...

Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment; the remaining groups were applied with para-chlorophenylalanine (PCPA...

In two different experiments, serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) levels were measured in rats, using HPLC with electrochemical detection, in 3 brain regions (hippocampus, cerebral cortex and hypothalamus) after acute i.p. treatment with diazepam (4 mg/kg), alprazolam (1.25 mg/kg) or vehicle. In the first experiment, rats received the acute treatment 30 min before they were...

Clobazam as a twice-a-day dosage (10 mg-20 mg) regimen and Diazepam in a thrice-a-day schedule (5 mg-5 mg-5 mg) were both effective in controlling moderate to severe anxiety neurosis. 83 patients were studied in a controlled, randomised, double-blind trial. Patients received active drug for the first six weeks and placebo for the next two weeks. Weekly evaluation was performed clinically for an...

Diazepam is metabolized by CYP2C19 and CYP3A4 in the liver. CYP2C19 shows genetic polymorphism associated with the poor metabolizer (PM) and extensive metabolizer (EM) phenotypes. The aim of this study was to assess the effect of diltiazem, a CYP3A4 inhibitor, on pharmacokinetics and dynamics of diazepam in relation to CYP2C19 genotype status. Thirteen healthy volunteers (eight EMs and five PMs...

[3H]Diazepam appears to bind specifically to a single, saturable, binding site located on rat brain membranes, with an affinity constant near 3 nM at pH 7.4. Specific binding constitutes more than 90% of total binding at 0 degrees and less than 10% of total binding at 37 degrees. Arrhenius plots suggest a sharp conformational change in the diazepam receptor near 18 degrees. Mitochondrial fracti...