A two-stage method for in vitro propagation of six Genista species from shoot tips was developed. Multiple microshoot cultures were obtained by growing the shoot tip explants on Schenk and Hildebrandt medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)-purine and 0.99 microM thidiazuron. The best shoot elongation was achieved on Schenk and Hildebrandt medium containing 4.92 ...

Cotton (Gossypium spp.) belonging to the genus Gossypium in the family Malvaceae. Cotton is an important fibre crop of global importance. It is grown in tropical and subtropical regions of more than 80 countries with an annual production of 20 million tonnes. It is an important source of oil and high quality protein meal and plays a significant role in the national economy. Among the cotton pro...

The effects o f thidiazuron (TDZ) on adventitious shoot bud formation from leaf and peti­ ole explants excised from in-vitro-grown plantlets o f a begonia were tested by a two-step procedure: a short exposure (5 to 120 min) to 40 pM TDZ in liquid MS medium followed by culture on agar-solidified MS medium without growth regulators. Exposure of explains for five min to TDZ induced bud formation, ...

An in vitro regeneration protocol was developed for Capsicum chinense Jacq. cv. Naga King Chili, a very pungent chili cultivar and an important horticultural crop of Nagaland (Northeast India). Maximum number of shoot (13 ± 0.70) was induced with bud-forming capacity (BFC) index of 10.8, by culturing nodal segments in Murashige and Skoog (MS) medium supplemented with 18.16 μM Thidiazuron (TDZ) ...

Effect of various concentrations of different sugars was investigated for induction of root and shoot from basmati rice. Explant, seeds (grains) were transferred in 1⁄2 MS basal media fortified with different concentrations of carbon source like sucrose, maltose and dextrose. In vitro regenerated rice plantlets were healthy and attained a length of 16.17cm at 0.25 % concentration within a week....

Pterocarpus santalinus belongs to fabaceae family and is one of the endemic and endangered medicinal plant species of seshachalam hills, Tirumala, Andhra Pradesh state, India. In the present study we have successfully demonstrated the in vitro regenartion of this plant through shoot bud culture. The seeds germinated sequentially on plastic trays and transferred polythene bags for 21 days served...

The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-...

In vitro propagation through nodal explants by indirect organogenesis of Clinacanthus siamensis Bremek. Nodal segments were surface sterilised in NaOCl for 5 min. This is followed by treating it with 0.1% Hgcl2 for 8 min. Different concentrations of BAP and 2P were added to full strength MS medium for culture initiation. Multiple shoots were formed in MS medium augmented with various concentrat...

The availability of a system for direct transfer of antifungal candidate genes into European chestnut (Castanea sativa Mill.) would offer an alternative approach to conventional breeding for production of chestnut trees tolerant to ink disease caused by Phytophthora spp. For the first time, a chestnut thaumatin-like protein gene (CsTL1), isolated from chestnut cotyledons, has been overexpressed...