Plants have a profound capacity to regenerate organs from differentiated somatic tissues, based on which propagating plants in vitro was made possible. Beside its use in biotechnology, in vitro shoot regeneration is also an important system to study de novo organogenesis. Phytohormones and transcription factor WUSCHEL (WUS) play critical roles in this process but whether and how epigenetic modi...

BACKGROUND Citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct productio...

An efficient protocol for direct shoot organogenesis has been developed for the medicinal plant Aerva lanata (L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.25-2.0 mg L(-1) thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organoge...

Medium composition for the callus induction, shoot multiplication in four Indian medicinal plants, Boerhaavia diffusa, Baliospermum axillare, Abutilon indicum and Nymphoides cristatum were standardized using Murashige and Skoog`s plant tissue culture medium as the basal medium. The protocol thus standardized was further evaluated replacing sucrose and vitamins with the fruit pulp of Borassus fl...

To develop an ef®cient procedure for plant regeneration from leaf-derived callus of Lavandula vera DC, the production of multiple shoots and the formation of roots from these shoots were studied. When calli were cultured in a medium with 4.0 10ÿ7 M N-(2-chloro-4-pyridyl)-N0phenylurea (CPPU), urea-type cytokinin, multiple shoots were obtained from the greenish surface of the callus ef®ciently at...

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...

An efficient embryogenic response (both direct and indirect) of two different varieties of sugarcane (CP 77,400 and BL-4) was obtained in this study. The results demonstrates that fresh inner whorl of leaves and shoot apical meristem of sugarcane were highly amenable to in vitro somatic embryogenesis (both direct and indirect). A combination of 3.0 mg/l 2,4-D with 0.25 mg/l BAP was more potent ...