In order to assess the contribution of 3.3',5-triiodo-L-thyronine (T(3)) to overall thyroid hormone economy, conjoint measurements of the kinetics of peripheral T(3) metabolism and the total concentration of T(3) in serum were made in a group of normal subjects and in a group of patients with hyperthyroid Graves' disease. As judged from the disappearance of trichloroacetic acid-precipitable (13...

The kinetics of conversion of dihydroxyacetone (DHA) to methylglyoxal (MGO) were investigated in mānuka honeys and DHA-doped clover honeys stored between 4 and 37°C. Both the disappearance of DHA and appearance of MGO were confirmed as overall, first order reactions, albeit probably composites of multiple reactions. Increasing the storage temperature accelerated the rate of DHA loss and the ini...

Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This was performed by fixing fused cells in different time intervals following the ...

Photosynthate translocation in single leaflets of healthy and curly top virus-infected tomatoes was investigated using (14)C as a marker. The amount of radioactivity found in plant parts not exposed to (14)CO(2) was substantially lower in diseased than in healthy plants. The time lag for the appearance of (14)C in the petiole was considerably longer in the infected plants than in the healthy. T...

We show that vimentin filaments are present in undifferentiated Friend murine erythroleukemia cells, but are lost progressively to undetectable levels by 96 h of dimethyl sulfoxide-mediated differentiation. The amount of newly synthesized cytoskeletal vimentin is decreased dramatically by 24 h of induction, and is paralleled by a rapid loss of vimentin mRNA (approximately 25-fold reduction at 9...

In humans, the monocyte pool comprises three subsets (classical, intermediate, and nonclassical) that circulate in dynamic equilibrium. The kinetics underlying their generation, differentiation, and disappearance are critical to understanding both steady-state homeostasis and inflammatory responses. Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge fir...

The kinetics of the equilibrium anionic polymerization of some cyclic siloxanes is modelled by using neural networks. Feedforward neural networks with one or two hidden layers have been used to appreciate the rates of disappearance of octamethylcyclotetrasiloxane and aminopropyl disiloxane at different catalyst concentrations (direct modelling). Alternatively, another neural model has been deve...

The kinetics of GSH oxidation to GSSG by cis-(diaqua)-bis-(ethylenediamine) cobalt(III) perchlorate was studied spectophotometrically under pseudo-first order condition using 10[Co(III)] = 5 mol dm, 2.5 ≤ 10[GSH] ≤ 10.00 mol dm, 3.5 ≤ pH ≤ 5.0, 318K ≤ T ≤ 333K at a fixed ionic strength I = 0.3 mol dm (NaClO4). The disappearance of [Co (III)] at 500 nm with time showed first-order kinetic trend....

CD4 and CD8 are thought to function as coreceptors by binding to the cognate major histocompatibility complex (MHC) molecules recognized by the T cell antigen receptor (TCR) and initiating the signal transduction cascade. We report that during T cell-antigen-presenting cell interaction, triggered TCRs and coreceptors are downregulated and degraded with identical kinetics. This coordinated disap...

As part of a mechanistic study of the electrooxidation of C1 molecules we have systematically investigated the dissociative adsorption/oxidation of formaldehyde on a polycrystalline Pt film electrode under experimental conditions optimizing the chance for detecting weakly adsorbed reaction intermediates. Employing in situ IR spectroscopy in an attenuated total reflection configuration (ATR-FTIR...