نتایج جستجو برای: dna amplification

تعداد نتایج: 547367  

Journal: :The Journal of Chemical Physics 2014

Journal: :Analytica Chimica Acta 2021

One challenge in point-of-care (POC) diagnostics is the lack of room-temperature methods for RNA detection based on enzymatic amplification and visualization steps. Here we perform reverse transcription lesion-induced DNA (RT-LIDA), an isothermal method that only requires T4 ligase. RT-LIDA involves RNA-templated ligation primers to form complementary (cDNA) followed by toehold-mediated strand ...

Journal: :Journal of the American Chemical Society 2020

Journal: :Proceedings of the National Academy of Sciences 1991

Journal: :Nucleic acids research 2001
G Nallur C Luo L Fang S Cooley V Dave J Lambert K Kukanskis S Kingsmore R Lasken B Schweitzer

While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the applicat...

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