Objective To assess the safety profile of lacosamide monotherapy in elderly (≥65 years) subjects with diabetic neuropathic pain (DNP). Methods Of 1,863 DNP subjects in double-blind, randomized, placebo-controlled trials of lacosamide monotherapy (NCT00861445, NCT00235469, NCT00238524, NCT00135109, NCT00350103), 502 were elderly. Safety data from elderly subjects were compared with that of you...

OBJECTIVE The mitochondrial uncoupling agent 2,4-dinitrophenol (DNP), historically used as a treatment for obesity, is known to cross the blood-brain-barrier, but its effects on central neural circuits controlling body weight are largely unknown. As hypothalamic melanocortin neuropeptide Y/agouti-related protein (NPY/AgRP) and pro-opiomelanocortin (POMC) neurons represent key central regulators...

Diabetic Nephropathy (DNP) is a chronic disease caused by diabetes that leads to end stage renal diseases. Although various pathological mechanisms have been proposed till date on the progression of DNP, the exact cause of this disease is still unknown. Here, we have focused on the modifications of low density lipoproteins (LDL) and its pathogenicity in DNP. LDL modification, specifically oxida...

The ability of guinea pigs to recognize poly-I,-lysine (PLL), and hapten conjugates of PLL (H-PLL) as antigens is under the control of a single autosomal dominant gene which we will refer to as the PLL gene (1-3). Responder animals carrying this gene form specific antihapten antibodies and display delayed hypersensitivity reactions after immunization with H-PLL conjugates such as 2,4-dinitrophe...

Dynamic Nuclear Polarization (DNP) enhances signal to noise in NMR experiments, by transferring the large electron Boltzmann polarization to nuclear polarization, via application of pulsed or continuous-wave microwave irradiation. This results in increases in NMR sensitivity of 2-3 orders of magnitude. DNP greatly reduces experimental times and makes some experiments possible that are otherwise...

Dynamic Nuclear Polarization (DNP) increases the sensitivity of NMR spectroscopy by using high frequency microwaves to transfer the polarization of the electrons to the nuclear spins. The enhancement in NMR sensitivity can amount to a factor of well above one hundred, enabling faster data acquisition and greatly improved NMR measurements. With the increasing magnetic fields (up to 23T) used in ...

The migration inhibitory factor (MIF) production of peripheral lymphocytes following exposure to dinitrophenylated microsomes derived from both human (DNP-hy-Mic) and guinea pig (DNP-gp-Mic) epidermis was quantitated to detect human contact sensitivity to dinitrochlorobenzene (DNCB). The lymphocytes from nonsensitized subjects did not generate MIF following exposure to either antigen. With DNP-...

B cell hyperactivity and resistance to tolerance induction are well-recognized immunologic abnormalities associated with both human and murine models of systemic lupus erythematosus. Studies evaluating the role of B cells in these defects have been complicated by the difficulties of consistently isolating large numbers of B cells from T cells and other host-derived regulatory factors. We have r...

This paper describes a method for the purification of monoclonal antibodies (rat anti-2,4-dinitrophenyl IgG: IgG(DNP); and mouse antidigoxin IgG: IgG(Dgn)) from ascites fluid. This procedure (for IgG(DNP)) has three steps: (i) precipitation of proteins heavier than immunoglobulins with ammonium sulfate; (ii) formation of cyclic complexes of IgG(DNP) by causing it to bind to synthetic multivalen...

AIM It is reported that ABCA1, which plays a key role in cholesterol transport and apolipoprotein E (APOE) metabolism in the brain, is related to Alzheimer's disease. However, few studies have focused on the relationship between the ABCA1 gene and the therapeutic response to donepezil (DNP), which has been shown to be related to reduced sAPP production.This study evaluated the association betwe...