A novel two-dimensional (2D) separation system for proteins was reported. In the system, a piece of dialysis hollow-fiber membrane was employed as the interface for on-line combination of capillary isoelectric focusing (CIEF) and capillary non-gel sieving electrophoresis (CNGSE). The system is similar equivalent to two-dimensional polyacrylamide gel electrophoresis (2D PAGE), by transferring th...

We demonstrate a two-dimensional (2D) separation microchip allowing capillary isoelectric focusing-capillary zone electrophoresis (cIEF-CZE) to be coupled to electrospray ionization mass spectrometry (ESI/MS). In the first dimension, separation is according to pI values (cIEF), while CZE occurs in the second dimension during mobilization towards the monolithically integrated ESI emitter. The se...

Jurkat leukemia cells induced to undergo apoptosis by treatment with an antibody against the Fas receptor have two annexin V (AV)-binding subpopulations: (a) single-positive cells that bind AV but not propidium iodide (PI); and (b) double-positive cells that bind AV and PI. The single-positive population is thought to represent an early stage of apoptosis. We have examined the relationship betw...

The solution of DGLAP evolution equation for the twist-3 gluon operators is obtained in the Double Logarithmic Approximation of QCD perturbation theory. The method used for the solution is similar to the reggeon field theory. The asymptotics of the twist-3 parton correlation function for the small Bjorken variables xB is found. 1. The growth of the structure function f(xB, Q ) in the region of ...

In order to overcome the limitations of carrier ampholyte generated pH gradients, IPGs were developed in the late 1970s. However, the 2-DE pattern we included in the first publication on IEF with IPGs [Bjellqvist et al., J. Biochem. Biophys. Methods 1982, 6, 317-339] was far from being competitive to O'Farrell's high-resolution 2-DE with carrier ampholytes. Our 2-DE pattern in this article was,...

The mechanism of the cytotoxic action of 2-chlorodeoxyadenosine in mouse FM3A cells was investigated. Imbalance of the dNTP pools occurred within 3 h of treatment with 20 microM 2-chlorodeoxyadenosine; the dATP and dGTP pools were depleted and the dTTP pool increased. 2-Chlorodeoxyadenosine added to the culture medium broke mature DNA strands, giving fragments of 100-200 kilobase pairs as found...

We provide a new way of simultaneously parametrizing arbitrary local CR maps from real-analytic generic manifolds M ⊂ <mml:mrow class="MJX-TeXAtom-OR...

The feasibility of capillary electrophoresis frontal analysis for the study of low-molecular-weight ligandchondroitin sulfate interactions was investigated. The interaction of the 7 cationic ligands (lidocaine, propranolol, scopolamine, N-methyl scopolamine, N-butyl scopolamine, 2-propylisochinolinium, and methyl viologen) with the glycosaminoglycan chondroitin sulfate was investigated in 0.067...

BACKGROUND: Clostridium septicum has played a significant role as a causative agent of many acute fetal diseases in man and animals. Alpha- toxin is the main factor in the pathogenesis of C. septicum with hemolytic, necrotic and lethal activities. OBJECTIVES: The study was designed to evaluate alpha-toxin purification and antibody production rate against a local strain of C. septicum NH2 which ...