Artificial cells are best defined as micrometre-sized structures able to mimic many of the morphological and functional characteristics of a living cell. In this mini-review, we describe progress in the application of droplet-based microfluidics for the generation of artificial cells and protocells.

Submit Manuscript | http://medcraveonline.com Abbreviations: PDA: Pancreatic Ductal Adenocarcinoma; CTCs: Circulating Tumor Cells; ctDNA: Circulating Tumor DNA; EXV: Extracellular Vesicles; NGS: Next Generation Sequencing; OS: Overall Survival; EpCAM: Epithelial-Cell Adhesion Molecule; NE-iFISH: Negative Enrichment Method Coupled to Immunofluorescence in Situ Hybridization; ddPCR: Digital Dropl...

Submit Manuscript | http://medcraveonline.com Abbreviations: PDA: Pancreatic Ductal Adenocarcinoma; CTCs: Circulating Tumor Cells; ctDNA: Circulating Tumor DNA; EXV: Extracellular Vesicles; NGS: Next Generation Sequencing; OS: Overall Survival; EpCAM: Epithelial-Cell Adhesion Molecule; NE-iFISH: Negative Enrichment Method Coupled to Immunofluorescence in Situ Hybridization; ddPCR: Digital Dropl...

A modified model has been analytically developed to describe the induction time of an elliptic air bubble in contact with an elliptic hydrophobic oil droplet. The role of hydrophobicity was revealed in the slippage of liquid over the surfaces of bubble and droplet. In this condition, the analytical relationships for pressure distribution and consequently hydrodynamic resistance force through th...

In this study, for the first time we report on a comprehensive overview of different strategies to hyphenate droplet-based sample handling and preparation with electrophoretic separation in formats (i.e. microchip capillary electrophoresis). Droplet-interfaced electrophoresis is an emerging technique which micro/nanometric droplets are used as bridge carrier target analytes between treatment el...

Due to the COVID-19 pandemic, research on quantitative evaluation of risk infection has become necessary. Because airborne transmission occurs by inhalation droplets from infected people, understanding mechanism droplet generation is important. In this study, size distribution produced various expiratory activities was investigated, results were compared with those previous studies, and applica...

Submit Manuscript | http://medcraveonline.com Abbreviations: PDA: Pancreatic Ductal Adenocarcinoma; CTCs: Circulating Tumor Cells; ctDNA: Circulating Tumor DNA; EXV: Extracellular Vesicles; NGS: Next Generation Sequencing; OS: Overall Survival; EpCAM: Epithelial-Cell Adhesion Molecule; NE-iFISH: Negative Enrichment Method Coupled to Immunofluorescence in Situ Hybridization; ddPCR: Digital Dropl...

This paper describes stepwise on-demand generation and fusion of femtolitre aqueous droplets based on interfacial tension. Sub-millisecond reaction times from droplet fusion were demonstrated, as well as a reversible chemical toggle switch based on alternating fusion of droplets containing acidic or basic solution, monitored with the pH-dependent emission of fluorescein.