To monitor nitrate and peptide transport activity in vivo, we converted the dual-affinity nitrate transceptor CHL1/NRT1.1/NPF6.3 and four related oligopeptide transporters PTR1, 2, 4, and 5 into fluorescence activity sensors (NiTrac1, PepTrac). Substrate addition to yeast expressing transporter fusions with yellow fluorescent protein and mCerulean triggered substrate-dependent donor quenching o...

Programmed -1 ribosomal frameshifting (-1 PRF) is a mechanism that directs elongating ribosomes to shift-reading frame by 1 base in the 5' direction that is utilized by many RNA viruses. Importantly, rates of -1 PRF are fine-tuned by viruses, including Retroviruses, Coronaviruses, Flavivriuses and in two endogenous viruses of the yeast Saccharomyces cerevisiae, to deliver the correct ratios of ...

OBJECTIVE To examine the effect of sedentary behavior on blood pressure (BP) in young children using different indicators of sedentariness. DESIGN Cross-sectional study. SETTING A rural Midwestern US community. PARTICIPANTS Children aged 3 to 8 years (N = 111). Intervention Adiposity was assessed using dual energy x-ray absorptiometry. Objective measurements of sedentary activity were obt...

Hypervideo based physiotherapy trainings bear an opportunity to support patients in continuing their training after being released from a rehabilitation clinic. Many exercises require the patient to sit on the floor or a gymnastic ball, lie on a gymnastics mat, or do the exercises in other postures. Using a laptop or tablet with a stand to show the exercises is more helpful than for example jus...

Wireless sensor networks are an essential tool for monitoring distributed remote environments and major difficulties are identifying the fault node. The extinction of damaged sensor measurements in WSNS will cause not only a dispossession of network quality of service, but also a huge load on the limited power. In this project proposes Diffusing Update Algorithm mapreading protocol to make cert...

BACKGROUND High-throughput methods based on molecular reporters have greatly advanced our knowledge of cell signaling in mammalian cells. However, their ability to monitor various types of cells is markedly limited by the inefficiency of reporter gene delivery. Recombinant adeno-associated virus (AAV) vectors are efficient tools widely used for delivering and expressing transgenes in diverse an...

A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead BacLight bacterial viability assay to mo...

The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1...

It is suggested to screen for bone metastases with bone scans and monitor for lymph node and visceral metastases/progression with imaging of the abdomen and chest. Patients with a rapid PSA doubling time (PSADT) (<8 months) are at risk for developing earlier metastases. Imaging in these patients should be performed every 3 to 6 months. Patients with slower PSA DT (>12 months) should be screened...

This paper describes a study of teacher scaffolding to support reflection and self-regulated learning (SRL) with an open learner model (OLM) in a geography based task on a touch screen. The study was carried out in 6 one-on-one sessions with students between the ages of 10 and 11. We present examples of teachers scaffolding students’ SRL behaviours using the OLM, demonstrating how an OLM can be...