Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envelopes (RSVE) was determined by quenching the fluorescence of calcein with cobal...

A fluorocarbon and hydrocarbon organic solvent mixture is known as a temperature-induced phase-separation solution. When a mixed solution of tetradecafluorohexane as a fluorocarbon organic solvent and hexane as a hydrocarbon organic solvent (e.g., 71:29 volume ratio) was delivered in a capillary tube that was controlled at 10°C, the tube radial distribution phenomenon (TRDP) of the solvents was...

INTRODUCTION The provision of an effective apical seal is an essential factor when choosing an appropriate material for success of root-end surgeries. Root-end resection, preparation and obturation should provide an adequate apical seal. The aim of this in vitro study was to investigate bacterial and dye microleakage of four different root-end filling materials and compare the efficacy of these...

the sscp technique is based on the appearance of new “refolding” conformations during electro-phoresis due to mutation. in order to develop a simple, non-radioactive sscp analysis method so that it can reliably detect single nucleotide changes in pcr products up to 500 bp in length, extensive optimisation trials were performed . the best separation of sscp bands of pcr products up to 500 bp in ...

BACKGROUND One ideal property of a root-end filling material is its apical sealing ability. The aim of this in vitro study was to assess bacterial and dye microleakage of white and gray mineral trioxide aggregate (WMTA and GMTA), Portland cement and calcium-enriched mixture (CEM) cement used as root-end filling material, and to assess the agreement between these two test methods. MATERIALS AN...

zno and tio2 nanoparticles wereimpregnated with merbromin dye and used as modified photocatalysts for degradation of phenol. dye-modified zno and tio2 showed significantly higher photocatalytic activity than neat zno and tio2 under visible light illumination. moreover, the prepared dye-modified zno showed superior photocatalytic efficiency in degradation of phenol compared to the dye-modified t...

The purpose of the present study was to find out if the differences in disappearance rates of T-1824, trypan blue, niagara sky blue and niagara sky blue 6B depend upon the binding of these dyes by the plasma proteins (Gregersen and Rawson, 1943). The affinity of the various dyes for the plasma proteins was investigated: 1, with the electrophoresis method of Tiselius (1937) ; 2, by the ultracent...

Educational laboratories require the availability of direct wet mount preparations that can last a long time with good quality. Various studies have mentioned techniques so time. This study aims to analyze composition dyes and glycerol in worm eggs on quality shelf life preparations. The solvent variations used for were Angkak 2%, eosin addition concentration 20%-45%. number treatments repetiti...

A novel electrophoretic separation technique is presented, where continuous demonstrated using free flowing liquid foams. Continuous foam electrophoresis combines the principle of capillary and interactions between analytes electrical double layer, with ability Free Flow Electrophoresis to continuously separate recover automatically. used provide a network deformable micro nano channels high su...