As axons advance to appropriate target tissues during development, their growth cones encounter a variety of cell adhesion molecules (CAMs) and extracellular matrix molecules (ECM molecules). Purified CAMs and ECM molecules influence neurite outgrowth in vitro and are thought to have a similar function in vivo. For example, when retinal ganglion cell (RGC) neurons are grown on different CAM and...

Sprouting angiogenesis is a dynamic process in which endothelial cells collectively migrate, shape new lumenized tubes, make new connections, and remodel the nascent network into a hierarchically branched and functionally perfused vascular bed. Endothelial cells in the nascent sprout adopt two distinct cellular phenotypes--known as tip and stalk cells--with specialized functions and gene expres...

The fluorescent lipophilic dye 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] was used to examine the distribution of membrane-bound organelles in growth cones of cultured rat sympathetic neurons. Within chemically fixed growth cones, intense DiOC6(3) fluorescence was localized predominately to the base or central region of growth cones. However, in most growth cones several thin DiOC6(3)-fluore...

In this research, modeling, simulation, and control of a methanol-to-olefins laboratory fixed-bed reactor with electrical resistance furnace have been investigated in both steady-state and dynamic conditions. The reactor was modeled as a one-dimensional pseudo-homogeneous system. Then, the reactor was simulated at steady-state conditions and the effect of different parameters including...

Individual tubular graphite cones (TGCs) are characterized using Raman spectroscopy at room temperature. A split G band at 1569 − 1587 cm is directly observed from the root to the tip of TGCs without requiring surface electromagnetic and chemical enhancement effects. The G band can be deconvoluted into two Lorentzian peaks at 1571 ± 2 and 1584 ± 3 cm, which may be attributed to the resonance en...

In this paper, a simple model for the prediction of centrifuge cone penetration tip resistance in sand is presented. The proposed method, which relates the tip resistance to the pressure to expand a spherical cavity, refines and simplifies Greeuw’s original equations. The method assumed the failure mode of a spherical cavity expansion pressure given as a function of shear stiffness, angle of fr...

Cone penetration resistance in sands with fines contents up to 35% is explored through drained cylindrical cavity expansion simulations with a modified MIT-S1 constitutive model. The constitutive model is calibrated to sands with 0%, 10%, 20%, and 35% low-plasticity fines with data from constant volume direct simple shear, drained and undrained triaxial compression, shear wave velocity, and 1D-...

We present a method to prepare tungsten tips for use in multi-tip scanning tunneling microscopes. The motivation behind the development comes from a requirement to make very long and conical-shape tips with controlling the cone angle. The method is based on a combination of a “drop-off” method and dynamic electrochemical etching, in which the tip is continuously and slowly drawn up from the ele...

Collapsin response mediator proteins (CRMPs) have been implicated in signaling of axonal guidance including Semaphorins. We have previously identified a unique member of this gene family, CRMP-associated molecule CRAM (CRMP-5) which is phylogenetically divergent from the other four CRMPs. In this study we have examined the distribution and function of CRAM in developing neurons. Immunohistochem...