Thirty postpubertal Holstein heifers were used to investigate the influence of dietary intake on 1) serum concentrations of progesterone (P4) and luteinizing hormone (LH) during diestrus and 2) estrous behavior. Heifers were randomly allotted to receive either 80, 100 or 120% of the National Research Council (NRC) requirements for energy, protein and dry matter intake for 139 d. Heifers were fe...

Among the gram-negative bacteria that cause mastitis, Escherichia coli are the most prevalent. The innate immune system provides initial protection against E. coli infection by detecting the presence of the foreign pathogens and by mounting an inflammatory response, the latter of which is mediated by cytokines such as IL1β, IL-8, and tumor necrosis factor (TNF)-α. Although changes in these cyto...

The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeate...

A series of experiments were carried out in order to determine doxycycline hydrochloride (DoxHCl) plasma levels in 6-wk-old turkeys medicated via drinking water containing DoxHCl at a concentration of 250 mg/L under laboratory and field conditions. Maximal plasma concentration (Cmax) values of 5.7 (+/-1.0) microgram/mL and 4.9 (+/-1.4) micrograms/mL obtained after DoxHCl administration during 2...

Studies were conducted in the period 2009-2011 in the experimental field of the Institute of Land Reclamation and Agricultural Mechanization – Sofia. The following treatments were used: a1 no herbicides with weeds (C0); a2 herbicide oxyfluorfen (Goal 2 E) at a rate of 250 ml / da; a3 herbicide oxyfluorfen (Goal 2 E) at a rate of 500 ml / da. The experiment was set up using long plots, three ver...

The fast development of multi-drug resistant (MDR) organisms increasingly threatens global health and well-being. Plant natural products have been known for centuries as alternative medicines that can possess pharmacological characteristics, including antimicrobial activities. The antimicrobial activities of essential oil (Calli oil) extracted from the Calligonum comosum plant by hydro-steam di...

The growing number of infections caused by multidrug-resistant pathogens has prompted a more rational use of available antibiotics given the paucity of new, effective agents. Monte Carlo simulations were utilized to determine the appropriateness of several doripenem dosing regimens based on the probability of attaining the critical drug exposure metric of time that drug concentrations remain ab...

This study aimed to evaluate the antimicrobial and antibiofilm activities of black pepper essential oil (BPEO) piperine effect on gene expression in a multispecies biofilm composed Listeria monocytogenes, Salmonella Typhimurium, Pseudomonas aeruginosa polypropylene surface. The minimal inhibitory concentrations BPEO were 100 25 mg/mL, respectively, against this consortium microorganisms. Sessil...

the aim of this study was to characterize the effect of typhoid fever on pharmacokinetic parameters of levofloxacin (lf) and compare the pharmacokinetic parameters of the said antibiotic in healthy human volunteers and patients with typhoid fever. total of 12 subjects were divided into two groups “a” (healthy volunteers) and “b” (typhoid patients). single oral dose of lf 500 mg was given and 5 ...

Background. Oxidative stress in schizophrenia may be caused partially by the treatment of patients with antipsychotics. The aim of the study was to establish the effects of polyphenol compounds derived from berries of Aronia melanocarpa (Aronox) on the plasma lipid peroxidation induced by ziprasidone in vitro. Methods. Lipid peroxidation was measured by the level of thiobarbituric acid reactive...