Enhanced Green Fluorescent Protein (eGFP) shows much stronger fluorescence than its ancestor, (GFP), thus has been widely applied as a reporter for biomedical research. In this study, we reported the expression of synthetic codon optimized gene encoding eGFP in Escherichia coli (E. coli). The was cloned into two vectors, pQE30 and pColdII resulting recombinant vectors were transformed E. M15 BL...

Abstract. Erviana R, Saengkun Y, Rungsa P, Jangpromma N, Mustofa, Daduang S. 2022. The recombinant expression and antimicrobial activity determination of Cecropin-like part Heteroscorpine-1 from Heterometrus laoticus. Biodiversitas 23: 5646-5653. Antimicrobial peptides are promising novel antibiotics that hold great potential in combating bacteria, fungi, viruses, parasites. Recent interest has...

UNLABELLED Objective(s) : Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity. MATERIALS AND METHODS The antigenic ...

Background: Fusion of the last two enzymes in pyrimidine biosynthetic pathway inverse order by having COOH-terminal orotate phosphoribosyltransferase (OPRT) and NH 2 -terminal orotidine 5′-monophosphate decarboxylase (OMPDC), as OPRT-OMPDC, has been described many organisms. Objective: The study aimed to select optimum host cell temperature for expressing recombinant fusion OMPDC-OPRT enzymatic...

BACKGROUND Solar energy is the ultimate energy source on the Earth. The conversion of solar energy into fuels and energy sources can be an ideal solution to address energy problems. The recent discovery of proteorhodopsin in uncultured marine γ-proteobacteria has made it possible to construct recombinant Escherichia coli with the function of light-driven proton pumps. Protons that translocate a...

Introduction Escherichia coli has a direct relationship with the diarrheagenic diseases, which occur quite in developing countries due to poor sanitation system and public health. It mainly affects children, causing a rate of morbidity and mortality worrying. This bacterium has a versatility of strains expressing different mechanisms and virulence factors, thus the importance in studying the ta...

BACKGROUND Escherichia coli is, to date, the most used microorganism for the production of recombinant proteins and biotechnologically relevant metabolites. High density cell cultures allow efficient biomass and protein yields. However, their main limitation is the accumulation of acetate as a by-product of unbalanced carbon metabolism. Increased concentrations of acetate can inhibit cellular g...

His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL2...