Membrane-bound particles called "quantasomes"' are found in shadowed2 or negatively stained3 chloroplast preparations examined in the electron microscope. The membrane-bound particles were considered to be integral components of the chloroplast electron transport system4 and were interpreted as morphological counterparts of photosynthetic units.5 Recently, it was demonstrated that the quantasom...

introduction: application of edta may increase the heavy metal availability and phytoextraction efficiency in contaminated soils. in spite of that, it might also have some adverse effects on soil biological properties. metals as freeions are considered to be severely toxic, whereas the complexed form of these metalswith organic compounds or fe/mn oxides may be less available to soil microbes. h...

Polyphosphate (polyp) kinase was prepared from EDTA-lysozyme spheroplasts of a Salmonella minnesota rough mutant. The enzyme, purified about 8o-fold, was free of ATPase and polyp-degrading activity and contained neither nucleic acids nor polyp which might act as primer. Using this enzyme, 32P-labelled polyp was prepared from [?P]ATP. The polyp was identified by the action of degrading enzymes, ...

First measurements of proton relaxation on chloroplast membranes are presented here. Experiments show that the water proton spin-lattice relaxation rate in chloroplast thylakoid membrane suspensions can be used to monitor membrane-bound manganese. The relaxation effect is reduced to 0.4 of its original value upon manganese extraction by washing with either alkaline Tris buffer or NH2OH/EDTA sol...

Calcium and EDTA interfere with radioimmunoassay of cAMP or acetylated cAMP. Both interferences with the assay of acetylated cAMP can be obviated by including in the buffer 15 mmol of CaCl2 per liter, which at the same time increases the affinity of the antibody by 89%. As a result, antibody can be decreased by 65%. This method requires no prepurification (extraction or chromatography), and no ...

Fractions enriched in Photosystem 1 or Photosystem 2 activity have been isolated from the cyanobacterium Chlorogloea fritschii after extraction of the membranes with digitonin and Triton X-100. Separation of the extract into the two components was achieved by using a Sepharose 6B column, calibration of which gave Kd values of 0.3 for the Photosystem 1 fraction and 0.53 for Photosystem 2. These ...

Final root canal irrigation stands as an effective strategy for eliminating the dentin infection. This study aimed to investigate and compare the antibacterial efficacy of QMix and other four final irrigation regimens in reducing Enterococcus faecalis within human root canals. Single-canal human teeth contaminated with E. faecalis for 4 weeks were prepared chemomechanically with sodium hypochlo...

EDTA, a common chelating agent, is becoming a major organic pollutant in the form of metal-EDTA complexes in surface waters, partly due to its recalcitrance to biodegradation. Even an EDTA-degrading bacterium, BNC1, does not degrade stable metal-EDTA complexes. In the present study, an ABC-type transporter was identified for possible uptake of EDTA because the transporter genes and the EDTA mon...

The performance of the m1000 system (Abbott Laboratories, Illinois) as a front-end extraction system for high-throughput "in-house" quantitative real-time PCR assays was analyzed and compared to that of manual extraction of plasma and serum samples (hepatitis C virus [HCV] and hepatitis B virus [HBV]) and EDTA-blood samples (cytomegalovirus [CMV] and Epstein-Barr virus [EBV]). Linearity of extr...