Though the use of mutants and specific inhibitors, polyamines (putrescine spermidine, and spermine) have been shown to be essential for normal growth and development of fungi, bacteria, green plants and mammals (1). In bacteria and plants, polyamine synthesis initially involves the production of putrescine via either the ornithine decarboxylase (ODC) or arginine ARAŞTIRMA Cilt 57, No 1, S : 19 ...

The polyamines are low molecular mass aliphatic amines that can be synthesized by both prokaryotic and eukaryotic cells. The most common are putrescine, spermidhe and spermine (Fig. I ). The intracellular concentrations of these polyamines vary greatly with the proliferative status of the cell, and also in response to a wide variety of stimuli [ 1-31, Induction of polyamine biosynthesis, a deli...

As is known, bacterial polyamines, which include cadaverine and putrescine, are capable of influencing the activity immunocompetent cells in many ways. In particular, this situation observed long-term inflammatory diseases, especially with intensive reproduction microorganisms producing polyamines. It interest to study production one main anti-inflammatory cytokines, IL-10, under influence For ...

Abstract—As the population ages, age-related cognitive impairments are becoming an increasingly pressing problem. Currently, role of polyamines (putrescine, spermidine, and spermine) in pathogenesis various origin is actively discussed. It was shown that content brain tissue decreases with age. Exogenous administration makes it possible to avoid impairment and/or influence pathogenetic processe...

Several lines of evidence have suggested, but not proved, that polyamines are associated with DMA in intact cells. In an attempt to investigate the roles of polyamines in gene-associated func tions, we examined the effects of polyamine depletion on the spontaneous mutation rate in a rat basophilic leukemia cell line. The frequency of 6-thioguanine-resistant mutant cells increased by approximate...

Ubiquitous in nature, polyamines are a group of aliphatic amines, cationic at neutral pH, that are essential for cell growth and viability. Because of their positive charge, polyamines are able to bind by electrostatic linkages to many cellular macromolecules, including DNA, RNA, and proteins (Kusano et al. 2008). Polyamines are involved in the regulation of a diverse range of vital cellular pr...

The purpose of the present study was to clarify the properties of amine oxidase partially purified from soybean seedlings, which the authors used as an enzyme sample for the assay of diamines (putrescine, cadaverine) and polyamines (spermidine, spermine). The enzyme activity was highest with cadaverine followed by putrescine, spermidine, L7-diaminoheptane and spermine. The optimum pH was 7.8 fo...

Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spermine were present in the yeasts, spermine was not detected in any of the filamentous fungi.

We developed a new simple enzymatic assay method for measuring urinary polyamines (total amount of putrescine, spermidine, and cadaverine), using an acylpolyamine amidohydrolase and a putrescine oxidase. First, conjugated polyamines (putres cine, spermidine, and cadaverine) in urine were hydrolyzed by incubation with an acylpolyamine amidohydrolase at 30°for 1 hr. Then, free polyamines were se...

It is well known that the addition of spermine or spermidine to culture medium containing ruminant serum inhibits cellular proliferation. This effect is caused by the products of oxidation of polyamines that are generated by serum amine oxidase. Among the products, we found that acrolein is a major toxic compound produced from spermine and spermidine by amine oxidase. We then analysed the level...