The transcriptional activities of c-Ets-1 and v-Ets and their functional interaction with the AP-1 factor c-Jun were investigated. Several recombinant Ets proteins were produced and purified either from bacteria or from insect cells. Plasmid DNAs that contained the polyoma virus enhancer Ets/AP-1 element were used as templates for in vitro transcription assays in the presence of HeLa nuclear ex...

Diphenylamines, Inhibitors, Photosynthetic Electron Transport A number of 16 substituted diphenylamines have been tested for their inhibitory activity on photosynthetic NADP reduction and photophosphorylation. The most active compounds exhibited p l50 values of 6.0 in photosynthetic electron transport and 6.8 in cyclic photophosphorylation, respectively. The inhibition site in electron flow of ...

The avian acute leukemia virus (E26) induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. The viral protein responsible for transformation is a gag-myb-ets fusion protein that is located in the nucleus of the transformed cells. The cellular homologue of v-ets (c-ets-1) is highly expressed in lymphoid cells and differs from the v-ets gene at...

The tumor suppressor PTEN possesses lipid and protein phosphatase activities. It has been well established that the lipid phosphatase activity is essential for its tumor-suppressive function via the phosphoinositide 3-kinase (PI3K) and Akt pathways. The precise role of the protein phosphatase activity is still unclear. In the current study, we demonstrate that overexpression of wild-type PTEN i...

4. CRANE, F. L., LESTER, R. L., WIDMER, C. and HATEFI, Y. Studies on the electron transport system XVIII. Isolation of coenzyme Q from beef heart and beef heart mitochondria. Biochim. Biophys. Acta 32: 73-79. 1959. 5. CRANE, F. L., WIDMER, C., LESTER, R. L. and HATEFI, Y. Studies on the electron transport system XV. Coenzyme Q (Q275) and the succinoxidase activity of the electron transport part...

Environmental stresses like temperature, high light, UV radiation and salt stress are known to affect the efficiency of photosynthesis (Stainer and Cohen –Bazire, 1977; Tandeau de Marsac and Houmard, 1993). Salt stress inhibits fundamental processes such as plant growth and productivity which are often associated with the decreased photosynthesis (Greenway and Munns,1980). A number of studies h...

Current policies in the road transport sector fail to deliver consistent and efficient incentives for greenhouse gas abatement (see companion article by Creutzig et al., in press). Market-based instruments such as cap-and-trade systems close this policy gap and complement traditional policies that are required where specific market failures arise. Even in presence of strong existing non-market ...

Fetal growth is critically dependent on energy metabolism in the placenta, which drives active exchange of nutrients. Placental oxygen levels are therefore vital, and chronic hypoxia during pregnancy impairs fetal growth. Here we tested the hypothesis that placental hypoxia alters mitochondrial electron transport chain (ETS) function, and sought to identify underlying mechanisms. We cultured hu...

Hypoxia-induced mitogenic factor (HIMF) possesses mitogenic, vasoconstrictive, angiogenic, and antiapoptotic effects. While HIMF is known to be expressed in developmental mouse lung, its gene expression regulation during this period is completely unknown. Genomic sequencing of HIMF gene has shown that there is an Ets-1 binding site in its 5'-promoter-region. To test the hypothesis that Ets-1 pr...