BackgroundThe retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate protective effect curcumin on rat (RRVECs) high glucose circumstance.ObjectiveThe cultured RRVECs were identified and characterized both vWF CD31 immunofluorescence expression. The activation ROS/NF-κB signal pathway was examin...

A new fault coverage test pattern generator using a linear feedback shift register (LFSR) called FC-LFSR can perform fault analysis and reduce the power of a circuit during test by generating three intermediate patterns between the random patterns by reducing the hardware utilization. The goal of having intermediate patterns is to reduce the transitional activities of Primary Inputs (PI) which ...

LFSR (Linear Feedback Shift Register) is commonly employed in various cryptography applications to generate pseudo-random numbers. The overall number of random state produced by the LFSR is determined by the feedback polynomial. LFSR is a shift register in which some of their outputs are taken in exclusive-OR format that forms the feedback path. So it capable to generate maximum of 2-1 random s...

A gel mobility retardation assay can be used to detect a protein-protein interaction. The assay is based on the electrophoretic mobility of a protein-protein complex being less than that of either protein alone. Electrophoretic mobility is detected by the fluorescence of a green fluorescent protein variant that is fused to one of the protein partners. The assay is demonstrated by using the inte...

We describe a platform for high-throughput electrophoretic mobility shift assays (EMSAs) for identification and characterization of molecular binding reactions. A photopatterned free-standing polyacrylamide gel array comprised of 8 mm-scale polyacrylamide gel strips acts as a chassis for 96 concurrent EMSAs. The high-throughput EMSAs was employed to assess binding of the Vc2 cyclic-di-GMP ribos...

The assembly and composition of human excision nuclease were investigated by electrophoretic mobility shift assay and DNase I footprinting. Individual repair factors or any combination of up to four repair factors failed to form DNA-protein complexes of high specificity and stability. A stable complex of high specificity can be detected only when XPA/RPA, transcription factor IIH, XPC.HHR23B, a...