The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...

Biologics manufacturing requires the clearance of Host Cell Proteins (HCPs) from recombinant therapeutic protein to acceptable low levels to ensure product purity and patient safety. To ensure adequate removal, a highly sensitive method, commonly in the form of Enzyme-Linked Immunosorbent Assay (ELISA), is necessary to quantify the HCPs amount in process intermediates and drug substance. We rep...

BACKGROUND In recent years, several new ELISAs for the detection of antibodies against the porcine reproductive and respiratory disease virus (PRRSV) in pig serum have been developed. To interpret the results, specificity and sensitivity data as well as agreement to a reference ELISA must be available. In this study, three commercial ELISAs (INgezim PRRS 2.0 - ELISA II, Priocheck® PRRSV Ab porc...

Background: An accessible and sensitive method for determining antibodies to a new coronavirus infection is often the key timely provision of necessary medical care patients with rheumatic diseases Objectives: Compare methods SARS-CoV-2 using rapid test enzyme-linked immunosorbent assay (ELISA) Methods: Methods an express (Chromatographic IgG / IgM (Xiamen Biotime Biotechnology, China)) by ELIS...

Aim. To compare two methods for the determination of N-terminal pro-brain natriuretic peptide (NT-proBNP) and soluble ST2 (sST2): rapid immunochemical standard enzyme immunoassay (ELISA), as well to determine possibility tests determining these biomarkers in acute myocardial infarction (AMI). Material . This open, non-randomized, single-center observational study included 41 patients: 20 with n...

Authors and Affiliations Colin C Collins, Stanislav V Volik, Anna V Lapuk, Yuwei Wang, Peter W Gout, Chunxiao Wu, Hui Xue, Hongwei Cheng, Anne Haegert, Robert H Bell, Sonal Brahmbhatt, Shawn Anderson, Ladan Fazli, Antonio Hurtado-Coll, Mark A. Rubin, Francesca Demichelis, Himisha Beltran, Martin Hirst, Marco Marra, Christopher A. Maher, Arul M. Chinnaiyan, Martin Gleave, Joseph R. Bertino, Mart...

In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, usin...

The matrix (M) protein of avian pneumovirus (APV) was evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of APV infection, a newly emergent disease of turkeys in United States. Sera from APV-infected turkeys consistently contained antibodies to a 30-kDa protein (M protein). An ELISA based on recombinant M protein generated in Escherichia...