New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation for grapes are based on the generation/induction of embryogenic callus cells that then transformed. Varieties such as Italia proven be very recalcitrant regeneration via somatic embryogenesis. In this work, develop...

Root-knot nematodes (RKNs; Meloidogyne spp.) induce feeding cells (giant cells; GCs) inside a pseudo-organ (gall) from still unknown root cells. Understanding GCs ontogeny is essential to the basic knowledge of RKN-plant interaction and to discover novel and effective control strategies. Hence, we report for the first time in a model plant, Arabidopsis, molecular, and cellular features concerni...

Abstract Background Barley is known to be recalcitrant tissue culture, which hinders genetic transformation and its biotechnological application. To date, the ideal explant for remains limited immature embryos; mechanism underlying embryonic callus formation elusive. Results This study aimed uncover different transcription regulation pathways between calli formed from (IME) mature (ME) embryos ...

Coffea arabica L. is a species of coffee that contribute for more than seventy percent world production. Various attempts have been made to obtain large quantities planting material through propagation in vitro somatic embryogenesis technology. The objective this experiment was evaluate the effect different plant growth regulators (PGRs) on callus induction (indirect embryogenesis) AS2K clone A...

Oil palm (Elaeis guineensis) is the highest oil-yielding commercially grown perennial tree. germplasm conservation and in vitro clonal propagation strengthened world’s efforts to ensure future food security. Cryopreservation provides long-term storage for germplasm. The of plant material at cryogenic temperatures (−196 °C) following dehydration causes cryoinjury. cryotolerance mechanism has rar...