the way to overcome current limitations in the generation of glucose-responsive insulin-producing cells is selective enrichment of the number of definitive endoderm (de) progenitor cells. sox17 is the marker of mesendoderm and definitive endoderm. the aim of the present research was to study the potential of sox17 knock-in cgr8 mouse embryonic stem (es) cells to differentiate into insulin produ...

the distribution, body size, and egg diameter and volume of ovigerous blue swimming crabs, portunus pelagicus , were examined according to habitat type and egg color in lasongko bay, central buton, indonesia between april 2013 and march 2014. ovigerous female crabs were sampled using gillnets and traps at seven stations. based on the results, the ovigerous female crabs with yellow to dark gray ...

background: human embryonic stem cells (hesc), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. in this study we examine the differentiating potential of hesc into hepatocytes by characterization of the expression of endoderm and liver-specific genes. methods: hesc were cultivated in suspension to form aggregates, the embryoid bodies. ...

CRISPR-Cas9 mutagenesis facilitates the investigation of gene function in a number developmental and cellular contexts. Human pluripotent stem cells (hPSCs), either embryonic or induced, are tractable model to investigate molecular mechanisms involved early human development cell fate decisions. hPSCs also have broad potential regenerative medicine model, investigate, ameliorate diseases. Here,...