We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability ...

Advanced maternal age and in vitro embryo production (IVP) predispose to pregnancy loss in horses. We investigated whether mare age and IVP were associated with alterations in mitochondrial (mt) DNA copy number or function that could compromise oocyte and embryo development. Effects of mare age (<12 vs ≥12 years) on mtDNA copy number, ATP content and expression of genes involved in mitochondria...

PURPOSE Femoral lengthening using a circular or mono-lateral frame is a commonly used technique. Fracture at the site of the regenerate bone is a major concern especially following removal of the external fixator. This aim of this study was to assess the rate of fracture of the regenerate bone in this single surgeon series of paediatric patients and determine potential risk factors. METHODS R...

Most of the cases of dental implant surgery, especially the bone defect extensively, are essential for alveolar ridge augmentation. As known as cell therapy exerts valuable effects on bone regeneration, numerous reports using various cells from body to regenerate bone have been published, including clinical reports. Mesenchymal cells that have osteogenic activity and have potential to be harves...

Background: Oocyte, embryo and ovarian tissue cryopreservation are proposed for fertility preservation to cancer patients who hope to be mothers after getting rid of the disease. Materials and Methods: Embryo cryopreservation is not possible for unmarried single girls without sperm partner and oocyte retrieval is a long time procedure. Thus ovarian tissue cryopreservation is suggested for ferti...

Soluble (SP), ionically bound (IBP) and covalently bound (CBP) peroxidases (POD) from artichoke leaves and heads have been characterized for the main biochemical parameters. The three PODs, in both leaves and heads, showed the major apparent catalytic efficiency (vmax,app/Km,app) towards ferulic acid, even though, in some cases, they showed higher affinity (Km,app) for other substrates. In leav...

Tadpoles of the bullfrog Rana catesbeiana lose the ability to regenerate limbs amputated through the femur at a very early stage in development. Well-differentiated limbs which in other species can regenerate if amputated through the ankle, cannot in this species. Nonregenerating tadpole limbs either show rapid healing of the cut surface by connective tissue with no breakdown of skeletal struct...