OBJECTIVE To analyze, through molecular biology, the diversity of the intestinal microbiota before and after resection of the ileocecal junction and reconstruction of intestinal transit with and without the creation of a neosphincter. METHODS Fourteen Wistar rats were divided into two groups: Group A (n = 7), submitted to resection of the ileocecal valve and end-to-end, single-layer ileocolic...

Abstract DNA-RNA hybrid structures have been detected at the vicinity of DNA double-strand breaks (DSBs) occurring within transcriptional active regions genome. The induction hybrids strongly affects repair these DSBs, but nature and how they are formed remain poorly understood. Here we provide evidence that R loops, three-stranded containing displaced single-stranded (ssDNA) can form sub-telom...

    Idiopathic colon perforation is rare in children. It is more common at the extremes of age. Splenic flextures, ileocecal and lower sigmoid regions are the most common sites of perforation. Delay in proper management of this condition is associated with high mortality and morbidity rate. We report on the case of a 2.5 years old girl who presented with fever, diarrhea, nausea and vomiting and...

The multifunctional Mre11-Rad50-Nbs1 (MRN) protein complex recruits ATM/Tel1 checkpoint kinase and CtIP/Ctp1 homologous recombination (HR) repair factor to double-strand breaks (DSBs). HR repair commences with the 5'-to-3' resection of DNA ends, generating 3' single-strand DNA (ssDNA) overhangs that bind Replication Protein A (RPA) complex, followed by Rad51 recombinase. In Saccharomyces cerevi...

background and objectives: postoperative ileus is common after abdominal resection. it causes gas store,distention, nausea, vomiting, and even pain. this study examines the impact of gum chewing as a type of sham feeding on the duration of postoperative ileus. methods: in this randomized control trial study, 70 patients in two groups (each group n=35) underwent abdominal resection. the a group ...