VELICHKOVA, E., M. YORDANOVA, M. BADALOVA, D. NIKOLOVA and Y. EVSTATIEVA, 2014. Adherence of Aspergillus awamori K-1 and Trichoderma viride SL-45 on loofa sponge for production of hydrolases. Bulg. J. Agric. Sci., Supplement 1: 97–99 In nature, fi lamentous fungi of the genus Aspergillus and Trichoderma are able to secrete a wide range of different enzymes into their environment. In recent year...

Immobilization of a phosphonated cobalt bis(terpyridine) catalyst on TiO2 nanoparticles generates photocatalyst that allows coupling aqueous CO2-to-syngas (CO and H2) reduction to selective oxidation biomass-derived oxygenates or cellulose formate. An enzymatic saccharification pre-treatment process is employed enables the use insoluble as an electron-donating substrate under benign conditions ...

The determination of cholesterol is greatly important because high concentrations this biomarker are associated to heart disease. Moreover, can be used as a fuel in enzymatic cells operating under physiological conditions. Here, we present biosensor and peroxide-free biofuel cell based on the electrocatalytic oxidation NADH generated during reaction dehydrogenase (ChDH) an alternative H2O2 bios...

In this study, cellulase was modified by immobilization technique using MIL-88B(Fe) as the carrier, and temperature, time, concentration pH of 30°C, 6 h, 1.2 mg/mL 5.0, respectively, were investigated optimal preparation conditions. Characterization analysis FT-IR, XRD TGA revealed that successful on did not disrupt integrity carrier lattice structure. Compared with free enzyme, Cellulase@MIL-8...

Abstract In order to develop new sustainable and reusable concepts for the degradation of omnipresent industrial plastics, immobilization (bio)catalysts on nanocarriers offers unique opportunities selective depolymerization catalyst recovery. this study, enzymes (lipase cutinase) were covalently immobilized carrier nanoparticles (SiO 2 Fe 3 O 4 @SiO ) through 3-(aminopropyl)trimethoxysilane glu...

A new support for the immobilization of β-d-galactosidase from Kluyveromyces lactis was developed, consisting mesoporous silica/titania with a chitosan coating. This presents high available surface area and adequate pore size optimizing efficiency enzyme and, furthermore, maintaining its activity. The obtained supported biocatalyst applied in hydrolytic activity tests o-NPG, showing 1223 Ug−1, ...

The highest specific activity for (C12O) enzyme purification was within the second stage of purificationsteps represented by precipitation with ammonium sulfate AS 2% (weight / volume), reaching about 14.56U. mg-1 after achieving 6.6 folds a recovery rate 78.3%. optimum catalyticactivity pH 6.5-8.5 and temperature 25 ° C. Immobilization of(C12O) biosilica diatom frustules DFS/G GA gave double s...

Since the discovery of enzymes as biological catalysts, study of their enormous catalytic power and exquisite specificity has been central to biochemistry. Nevertheless, there is no universally accepted comprehensive description. Rather, numerous proposals have been presented over the past half century. The difficulty in developing a comprehensive description for the catalytic power of enzymes ...

Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. This chapter covers the basic principles of enzymology, such as classification, structure, kinetics and inhibition, and also provides an overview of industrial appl...