onion (allium cepa) contains high levels of flavonoids. although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. fresh filtered juice of onion was prepared and its inhibitory effect on guinea ...

Supramolecular multilayer structures comprised of glucose oxidase (GOx), and Os complex derivatised poly(allylamine) (PAH-Os) have been built by alternate layer-by-layer (LBL) electrostatic adsorption in a self-assembly process. The resulting modified electrodes with integrated mediator were tested as reagentless glucose biosensors. The enzyme kinetic parameters and the surface concentration of...

Coenzymes participate in many of the enzyme analyses performed in the clinical laboratory. Supplementation of assay systems with optimal levels of coenzymes has recently been recommended as part of efforts to achieve interlaboratory standardization of enzyme measurements. Aspartate aminotransferase and alanine aminotransferase require pyridoxal phosphate for expression of enzyme activity. The r...

The determination of mercury(II) ions at the trace level by inhibition of the invertase enzyme-catalysed hydrolysis of sucrose into glucose and fructose coupled to electrochemical batch injection analysis was investigated using two approaches. In the first, the glucose produced was detected by injection of 100 microliters samples into the batch injection cell containing a platinum electrode mod...

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...

A dual-enzyme immunoconjugate system was evaluated for its cytotoxic effect on Candida tropicalis. Glucose oxidase, which generates hydrogen peroxide in the presence of glucose and oxygen and myeloperoxidase, which catalyzes the oxidation of halides in the presence of hydrogen peroxide, were each conjugated to a C. tropicalis-specific monoclonal antibody. Neither the glucose oxidase nor the mye...

WALDMAN, R. H., AND HELEN B. BURGH. Rapid methodfor study of enzyme distribution in rat kidney. Am.J. Physiol. 204(5) : 749-752 1963.-A rapid method for localizing enzymatic levels in various structures of the kidney is described. This method consists of dissecting a cone from rapidly frozen kidney, and measuring the enzyme activities of thin slices from the cortex to the papilla. The use of th...

Identification, monitoring and quantification of biomolecules in the CNS is a field of growing interest for identifying biomarkers of neurological diseases. Implantable enzymatic microelectrode biosensors are a unique minimally invasive tool for real-time monitoring. Progress in microfabrication technologies has opened new possibilities in the preparation of complex biosensor architectures with...

Qualitative and quantitative analysis of the urine for glucose is gener ’ .ly accepted as a useful measure for the detection of diabetics and for ihe daily follow up of those with proved diabetes as a rough practical estimation of the degree of control of blood sugar levels. In the past the test :ommonly employed using Benedict’s Solution depended on the fact that cupric sulfate was reduced in ...