This paper describes an electrochemical enzyme-linked immunosorbent assay (ELISA) on paper-based microfluidic devices. This paper-based immunoassay method has several advantages over conventional ELISA, such as short assay time, low cost, and high sensitivity. The limit of detection (LOD) of rabbit IgG used as a model system is ~ 3.9 fM; this value is lower than the LOD of paper-based colorimet...

CITE enzyme-linked immunosorbent assay (ELISA) was an unacceptable screening test for failure of passive transfer because of poor sensitivity. SNAP ELISA and glutaraldehyde coagulation tests are more appropriate screening tests. Specificity of the SNAP ELISA is better than the glutaraldehyde coagulation test. A second confirmatory test should be considered when using the glutaraldehyde coagulat...

Plant diseases caused by numerous pathogens such as bacteria, viruses, and fungi are responsible for substantial economic losses in the agricultural industry worldwide. Specific, sensitive, efficient diagnostic tools have been developed worldwide to mitigate prevent pathogenic threat. The revolutionized from classical methods more advanced molecular approaches enzyme-linked immunosorbent assay ...

An enzyme-linked immunosorbent assay (ELISA) using commercially available reagents was compared with standard serological tests for determination of antibody titers in patients with acute measles. The measles-specific ELISA was as sensitive as complement fixation, hemagglutination inhibition, and indirect fluorescent-antibody methods. The ELISA method used was simple, sensitive, inexpensive, an...

Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity.

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of Pasteurella multocida. The sandwich ELISA is based on two different murine monoclonal antibodies with specificity for the P. multocida toxin. The ELISA, which is now used as a routine test in Denmark, has several advantages compared with previously descri...

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

Tomato black ring virus (TBRV) and beet ringspot (BRSV) are closely related but distinct members of subgroup B the genus Nepovirus. Both viruses have broad host ranges transmitted by seed, pollen, ectoparasitic nematodes. Although 13 TBRV 3 BRSV genome sequences were already available, no attempt has been made to link sequence data from these recent with those historical isolates studied in pre...