Molecular detection of bacterial pathogens based on LAMP methods is a faster and simpler approach than conventional culture methods. Although different LAMP-based methods for pathogenic bacterial detection are available, a systematic comparison of these different LAMP assays has not been performed. In this paper, we compared 12 in-house real-time LAMP assays with a commercialized kit (Isotherma...

Straight cylindrical rods, 1.1–1.5 " 2.0–6.0 lm, occurring singly or in pairs. Conform to the general definition of the family Enterobacteriaceae. Gram negative. Motile by peritrichous flagella or nonmotile. Aerobic and facultatively anaerobic having both a respiratory and a fermentative type of metabolism, but anaerogenic biotypes occur. Oxidase negative. Chemoorganotrophic. Both acid and gas ...

Recently, the effects of antimicrobial activity of silver nanoparticles was considered by researcher because of Bacterial resistance to antimicrobial agents in chemical.The aim of this study was to evaluate the antimicrobial activity of the gold nanoparticles on Escherichia coli. Gold nanoparticles are chemically synthesized. Standard strain of E. coli was cultured in a nutrient broth. Minimum ...

Recently, the effects of antimicrobial activity of silver nanoparticles was considered by researcher because of Bacterial resistance to antimicrobial agents in chemical.The aim of this study was to evaluate the antimicrobial activity of the gold nanoparticles on Escherichia coli. Gold nanoparticles are chemically synthesized. Standard strain of E. coli was cultured in a nutrient broth. Minimum ...

aim : the purpose of this study was to investigate the prevalence of enteropathogenic escherichia coli (epec) and shiga toxin producing e. coli (stec) strains in healthy broilers in iran.   background : stec and epec strains as diarrheagenic e. coli are among the most prevalent causative agents in acute diarrhea. domestic animals, mainly cattle and sheep, have been implicated as the principal r...

Escherichia coli is one of the predominant species of facultative anaerobes in the human gut and usually harmless to the host; however, a group of pathogenic E. coli has emerged that causes diarrheal disease in humans. Referred to as Diarrheagenic E. coli (28) or commonly as pathogenic E. coli, these groups are classified based on their unique virulence factors and can only be identified by the...

Escherichia coli strains of serotype O51:H40 were studied with regard to the presence of several virulence properties and their genetic diversity and enteropathogenicity in rabbit ileal loops. This serotype encompasses potential enteropathogenic strains mostly classified as being atypical enteropathogenic E. coli (EPEC) strains, which are genetically closer to enterohemorrhagic E. coli than to ...

the aim of this study was to assess the prevalence of esbl-producing e. coli in agricultural fields of gorgan district, golestan province. soil samples were collected from 20 agricultural fields, half of them treated with chemical fertilizers and the rest with bovine manure. two samples, from surface and a depth of 15cm, were taken. after enrichment and culture onto macconkey agar, pink colored...

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance have made the expression of stable and active Pseudomonas protein possible in bot...

recently, the effects of antimicrobial activity of silver nanoparticles was considered by researcher because of bacterial resistance to antimicrobial agents in chemical.the aim of this study was to evaluate the antimicrobial activity of the gold nanoparticles on escherichia coli. gold nanoparticles are chemically synthesized. standard strain of e. coli was cultured in a nutrient broth. minimum ...