Background and Aim: Diabetes is a common disorder worldwide, and exhaustive efforts have been made to cure this disease. Gene therapy has considered as a potential curative method that has more stability in comparison with the other pharmaceutical methods. However, the application of gene therapy as a definitive treatment demands further investigation. This study aim is to prepare a suitable hi...

Abstract The mRNA epitranscriptome imparts diversity to gene expression by installing chemical modifications. Advances in detection methods have identified modifications eukaryotic, bacterial, and viral messenger RNAs (mRNAs). biological functions of mRNAs still remain be understood. Chemical are introduced synthetic meant for therapeutic applications maximize from the evade host immune respons...

The conserved acetyltransferase Gcn5 is a member of several complexes in eukaryotic cells, playing roles regulating chromatin organization, gene expression, metabolism, and cell growth differentiation via acetylation both nuclear cytoplasmic proteins. Distinct functions have been revealed through combination biochemical genetic approaches many vitro studies model organisms. In this review, we f...

Context:MicroRNAs (miRNAs) are a class of short, endogenously-initiated, non-coding RNAs that post-transcriptionally control gene expression via translational repression or mRNA turnover. MiRNAs have attracted much attention in recent years as they play critical roles in gene expression and are promising tools with many biotech and therapeutic applications. The molecular mechanisms und...

Background: Human chorionic gonadotropin (hCG) is a member of glycoprotein hormones family consist of two different non-covalently heterodimeric chains: alpha and beta subunits with 92 and 145 amino acids respectively. This hormone plays an important role in human reproduction and physiology especially for maintenance of the corpus luteum during the first months of pregnancy Materials and Metho...

Filamentous fungi are important model systems for understanding eukaryotic cellular processes, including the study of protein expression. A salient feature of fungi is the ability of the protein-processing machinery to perform all of the extensive posttranslational modifications needed in the complex world of eukaryotic organisms, making them great hosts for production of eukaryotic proteins. I...

Background: Ovine follicle stimulation hormone (OFSH) is a pituitary glycoprotein and belongs to the family of glycoprotein hormones. This hormone plays a key role in the function of the reproductive system: it is essential for sertoli cell function and spermatogenesis in testis and it stimulates the growth of ovulatory follicles in females. Ovine FSH hormone is a heterodimeric hormone consisti...

Over the past decade variety of hosts and vector systems for recombinant protein expression has increased dramatically. Researchers now select from among mammalian, insect, yeast, prokaryotic hosts, number vectors available use in these organisms continues to grow. With availability cDNAs coding sequencing information, it is certain that other, yet be developed will important future. Despite de...

context:micrornas (mirnas) are a class of short, endogenously-initiated, non-coding rnas that post-transcriptionally control gene expression via translational repression or mrna turnover. mirnas have attracted much attention in recent years as they play critical roles in gene expression and are promising tools with many biotech and therapeutic applications. the molecular mechanisms underlying t...

background: it seems that the success of vaccination for cancer immunotherapy such as dendritic cell (dc) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory t cell is the common factor. foxp3 transcription factor is the most specific marker of regulatory t cells. in different studies, targeting an immune response against regulat...