A membrane lipid abnormality induced by sickling and found as a permanent alteration in the irreversibly sickled cell (ISC) is the rearrangement of phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS) from the inner to the exterior side of the lipid bilayer. Since PS can provide a catalytic surface for the binding of blood coagulation factors and thus can exhibit procoagulant activity, w...

OBJECTIVE To investigate the presence of IgG and IgM types of anticardiolipin (aCL) and antiphospholipid (aPL) antibodies in younger Iranian patients with ischemic stroke. METHODS Both IgG and IgM types of aPL (cardiolipin, anti phosphatidyl inositol, anti phosphatidyl serine, anti phosphatidic acid and beta 2-glycoprotein I [B2-GPI]) and aCL alone (cardiolipin and B2-GPI) were measured in 11...

Alamethicin appears to allow voltage-dependent lipid exchange ("flip-flop") between leaflets of a planar bilayer. In membranes with one leaflet of phosphatidyl serine and one of phosphatidyl ethanolamine, the shape of the nonactin current-voltage curve accurately reports the difference in surface potential between the two sides of the membrane. The surface potential is itself a good measure of ...

The turnover of the four major erythrocyte phospholipids has been studied with (32)P, both in vivo and in vitro, in man and the dog. Phosphatidyl serine and phosphatidyl ethanolamine appeared to be stable erythrocyte lipids in both species. Turnover of the phosphate moiety of lecithin and sphingomyelin in the circulating erythrocytes of these two species seems entirely due to an exchange of the...

Silicic acid chromatography was employed to determine the distribution of the phosphatides in mouse epidermis undergoing normal growth changes induced by the hair growth cycle. These included epidermis removed 4, 12, and 22 days after plucking of the hair in both sexes of mice. Furthermore, the distribution of phosphatides was determined in mouse epidermis made hyperplastic by three, eighteen, ...

Work from this laboratory has established that brain cephalin (1, 2) is a mixture of three different phosphatides; namely, phosphatidyl serine (3), phosphatidyl ethanolamine, and diphosphoinositide. In a preliminary note (4) it has been shown that diphosphoinositide has inositol metadiphosphate’ as a constituent. The present paper contains detailed proof of this statement and reports some other...

The methylation steps in the biosynthesis of phosphatidylcholine by castor bean (Ricinus communis L.) endosperm have been studied by pulse-chase labeling. Endosperm halves were incubated with [methyl-(14)C]S-adenosyl-l-methionine, [2-(14)C]ethanolamine, [(14)C]ethanolamine phosphate, or [(14)C]serine phosphate. The kinetics of appearance were followed in the free, phospho-, and phosphatidyl-bas...

A strain of Escherichia coli bearing a hybrid plasmid containing the psd gene, starved for isoleucine by the addition of valine, produces amounts of phosphatidyl-serine decarboxylase, a membrane-bound enzyme, about 40-fold higher than wild type. At least 98% of the enzyme from cells with high levels of decarboxylase is isolated in the inner, cytoplasmic membrane fraction if the cells are broken...

The effect of peroxidative stress on tissue was studied by exposure of red blood cells (RBC) from patients with abetalipoproteinemia to minute amounts of H(2)O(2)in vitro. Red blood cells from untreated patients showed a marked sensitivity to H(2)O(2), as evidenced by hemolysis and lipid peroxidation (peroxidative hemolysis). The appearance of lipid peroxidation products in sensitive cells afte...

Major depressive disorders (MDDs) are often associated with a deficiency in long-chain omega-3 polyunsaturated fatty acids (?-3 PUFAs), as well signs of low-grade inflammation. Epidemiological and dietary studies suggest that high intake fish, the major source ?-3 PUFAs, is lower rates MDDs. Meta-analyses randomized placebo-controlled PUFAs intervention-trials primarily eicosapentaenoic acid (E...