Crispene E, a new clerodane-type diterpene, inhibited STAT3 dimerization in a cell-free fluorescent polarisation assay and was found to have significant toxicity against STAT3-dependent MDA-MB 231 breast cancer cell line and selectively inhibited the expression of STAT3 and STAT3 target genes cyclin D1, Fascin and bcl-2. Molecular docking studies suggest the molecule inhibits STAT3 by interacti...

While actin bundles are used by living cells for structural fortification, the microscopic origin of the elasticity of bundled networks is not understood. Here, we show that above a critical concentration of the actin binding protein fascin, a solution of actin filaments organizes into a pure network of bundles. While the elasticity of weakly cross-linked networks is dominated by the affine def...

Submit Manuscript | http://medcraveonline.com Abbreviations: ACTG1: Actin Gamma 1; ARHGDIA: Rho-GDP Dissociation Inhibitor Alpha; BDMR: Brachydactyly-Mental Retardation Syndrome; CMS: Chromosomal Microarray; CNV: Copy Number Variant; FISH: Fluorescence in situ Hybridization; FSCN2: Fascin Actin-Bundling Protein 2, Retinal; TWIST2: Twist-related Protein 2; HDAC4: Histone Deacetylase 4; GPC1: Gly...

While cells make use of many actin binding proteins (ABPs) simultaneously to tailor the mechanical properties of the cytoskeleton, the detailed interplay of different ABPs is not understood. By a combination of macrorheological measurements and confocal microscopy, we show that the ABPs fascin and filamin modify the structural and viscoelastic properties of composite in vitro actin networks ind...