Telomerase activity was measured with a quantitative assay, based on a modification of telomeric repeat amplification protocol method, in bladder cancers and apparently normal mucosa in 33 patients. In the same patients, the enzyme was also measured in exfoliated cells collected both with voided urine and bladder washings. Results obtained in urine were compared with those from 20 healthy subje...

Background: This study compared postvoid residual (PVR) urine volume by ultrasonography in pelvic organ prolapse (POP) patients before and after reduction to evaluate the need for accurately assessing PVR women with POP. Methods: was a prospective including 128 patients. Both standard methods measuring volume, urethral catheterization, portable abdominal ultrasound machines were used. An examin...

BACKGROUND Voiding before discharge is usually required after outpatient epidural or spinal anesthesia because of concern about bladder overdistention and dysfunction. Shorter duration spinal and epidural anesthesia may allow return of bladder function before overdistention occurs in low-risk patients (those younger than age 70, not having hernia, rectal, or urologic surgery, and without a hist...

Abstract Background Urine analysis is one of the most common tests for assessing urinary-tract and kidney diseases. In recent years several automated instruments examining urine have been introduced. Though introduction analyzers expected to reduce labour involved, turnaround time potential assay variations, manual remains gold standard or reference method urine. The new Sysmex UN-Series believ...

CONTEXT The identification of new molecular markers is one of the most challenging goals for the early detection of bladder cancer because available noninvasive methods have neither sufficient sensitivity nor specificity to be acceptable for routine use. OBJECTIVE To develop a relatively simple, inexpensive, and accurate test that measures telomerase activity in voided urine to apply to large...

OBJECTIVE In a trial to improve the diagnostic efficacy of conventional urine cytology we determine survivin RNA and matrix metalloproteinase 2 and 9 in urine of bladder cancer cases. METHOD Voided urine specimens were collected from patients with histologically confirmed bladder urothelial carcinoma (Group 1; n=46), urological patients without urothelial carcinoma (Group 2; n=20), and health...

BACKGROUND In urine, factors such as timing of voids, and duration at room temperature (RT) may affect the quality of recovered protein and metabolite data. Additives may aid with detection, but can add more complexity in sample collection or analysis. We aimed to identify the optimal urine processing protocol for clinically-obtained urine samples that allows for the highest protein and metabol...

PURPOSE To improve the specificity in prostate cancer diagnosis and to prevent unnecessary prostate biopsies, especially in the serum prostate-specific antigen (PSA) "gray zone" between 3 and 15 ng/mL, the implementation of prostate cancer-specific markers is urgently needed. The recently discovered prostate cancer antigen 3 (PCA3) is such a promising prostate cancer marker. In a previous singl...

Aims To determine whether the amount of ATP, prostaglandin E2 (PGE2), and acetylcholine (ACh) in voided urine are influenced enough by that released within lower urinary tract (LUT) for them to be useful biomarkers bladder function. Methods Participants without LUT symptoms collected total voids at 15, 30, 60, 120 min (20 males/23 females) 240 (18 males/26 following previous void. Aliquots were...