We used denaturing gradient gel electrophoresis (DGGE) to compare bacterial profiles in periodontium and root canals of teeth with combined periodontal-endodontic lesions. Samples of dental plaque and necrotic pulp were collected from thirteen extracted teeth with advanced periodontitis. Genomic DNA was extracted for polymerase chain reaction (PCR) analysis using universal bacterial primers. Th...

INTRODUCTION The aim of this study was to investigate the prevalence, phenotype, and genotype of Enterococcus faecalis isolated from saliva and root canals in patients with endodontic treatment failure. METHODS Samples were collected from 32 adults undergoing retreatment for periapical lesions after endodontic treatment performed at least 2 years previously. Isolates that were presumed to be ...

Aim: The aim of this study was to investigate the variations in root canal morphology of the Mandibular premolars in the population of Uttarakhand using periapical radiographs. Objective: For the success of the endodontic treatment, shaping and sealing of the root canals in the three dimension is mandatory, for which the thorough understanding of the root canal system and its variation is impor...

most mandibular second molars have one and two canals in distal and mesial roots, respectively. this report represents four cases of mandibular second molars with a single mesial and two distal root canals with two different canal configurations. after access cavity preparation, two teeth had one distal and two mesial orifices, whereas in the two other teeth one mesial and two distal orifices w...

AIM: To monitor chlorine consumption from nonagitated aqueous sodium hypochlorite (NaOCl) solutions in human root canals using a recently developed assay, which can determine the order of magnitude of available chlorine in small volumes of liquid. METHODOLOGY: The root canals of 80 extracted single-rooted human teeth were instrumented to ProTaper Universal F4 and irrigated using 1% NaOCl. Subse...

OBJECTIVE To determine the internal root morphology and gender variations in mandibular first permanent molars in a Kenyan population. DESIGN In vitro descriptive cross sectional study. SETTING School of Dental Sciences, University of Nairobi RESULTS The mesial root of mandibular first molars had two canals in 96.3% of the teeth in both males and females and-type IV canal configuration wa...

INTRODUCTION The aim of this study was to compare centering ability and dentin removal of three rotary systems in curved root canals of extracted teeth. MATERIALS AND METHODS Sixty root canals of mandibular first molars with curvatures ranging between 25-35(o) were divided into three groups of 20 teeth each. Based on pre-instrumentation radiographs that assessed the angle and the radius of ca...

introduction: the purpose of this study was to determine the effects of citrus aurantifolia (ca) extract on smear layer removal in different parts of root canals. materials and methods: thirty-nine single-rooted human teeth were randomly divided into three experimental ( n =12) and one control ( n =3) groups. teeth were instrumented using mtwo rotary instruments. root canals were irrigated with...

The endodontic treatment of maxillary molar with an aberrant root canal morphology can be diagnostically and technically challenging.1 Unusual root canal morphology in multirooted teeth is a constant challenge for diagnosis and successful endodontic treatment. Presence of extra canals, lateral canals, deltas is commonly encountered.2 This case report is presented to illustrate and describe the ...

Anatomical variations in root canal morphology are an enigma and it is this variability, which is often a complicating factor in a successful root canal treatment. To achieve success in endodontic therapy it is imperative that all the canals are located, cleaned and shaped and obturated three dimensionally. Maxillary first premolar having three separate roots has an incidence of 0.5-6%. Even ra...