Macrolides, one of the most commonly used class of antibiotics, are a group of drugs produced by Streptomyces species. They belong to the polyketide class of natural products. Their activity is due to the presence of a large macrolide lactone ring with deoxy sugar moieties. They are protein synthesis inhibitors and broad-spectrum antibiotics, active against both gram-positive and gram-negative ...

Bacteria used in self-healing of concrete crack need to be embedded into the concrete in the form of dormant spores. However, quantification of viable spores is still a challengeable task due to the lack of rapid, efficient and accurate methods. Considering that viable spores have a higher refractive index than vegetative cells and the refractivity issubject to decrease during the germination p...

in this research, a fast colorimetric method for the detection of 4-phenylthiosemicarbazid (4-ptsc) in environmental samples, is reported for the first time. semicarbazide compounds have been used as pesticides or fungicides in agriculture and for chemical synthesis in industrial processes. with this method, one or the total amount of semicarbazide in real samples can be detected within 6-7 min...

a novel, simple and more sensitive spectrophotometric procedure has been developed for the determination of brilliant blue fcf and carmoisine in food samples by an aqueous two-phase system (atps). after extraction, the absorbance values were measured at 527 and 632 nm for carmoisine and brilliant blue fcf, respectively. the effects of different parameters such as, polyethylene glycol (type and ...

An automated system for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding program. The system includes two high performance liquid chromatography (HPLC) pumps, an autosampler, a column heater, a spectrophotometric detector, and an integrator. With this new method, human error can be eliminated with great repeatability (CV ˆ 0.9%), and we can ru...

One titrimetric and two spectrophotometric procedures have been reported for the determination of albendazole and its tablets. Using titrimetry, the drug was titrated directly with Chloramine-T under acidic conditions using a Methyl Orange indicator. The spectrophotometric procedures involve treating the sample solution with a measured excess of Chloramine-T in an acid medium, followed by an es...

Absorption spectrophotometry has been and still is the industry standard for detection in HPLC. Limit of detection (LOD) and linear dynamic range (LDR) are the primary performance requirements and have driven continuous improvement of spectrophotometric HPLC detectors. Recent advances in HPLC column technology have led to low flow-rate HPLC such as capillary HPLC and nanoflow HPLC and put highe...

UV, first, second and third derivative spectrophotometric methods have been developed for the determination of ezetimibe in pharmaceutical formulation. The solutions of standard and sample were prepared in methanol. For the first method, UV spectrophotometry, the quantitative determination of the drug was carried at 233 nm and the linearity range was found to be 6-16 mug/ml. For the first, seco...

A simple and sensitive spectrophotometric method is proposed for the determination of palladium (II) in ppm level using 4-amino-3-mercapto-6-methyl-1, 2, 4-tirazine (4-H)-5-one, a nitrogen, sulphur and oxygen donor triazine, as the spectrophotometric reagent. In aqueous medium at a pH of 11, Pd(II) forms a light yellow colored complex with the proposed spectrophotometric reagent. The optimum co...

This work is concerned with the development and validation of two accurate, precise and specific spectrophotometric and chromatographic methods for analysis of Phenylepherine (PE), Lidocaine (LC) and Thiomersal (TM) in their ternary mixture and in Chromoderm® solution. The developed spectrophotometric method is the mean centering of ratio spectra (MCR) spectrophotometric method that depends on ...