Zhdanov, Victor M. (The D. I. Ivanovsky Institute of Virology, Moscow, USSR), Nonna B. Azadova, and Leonid V. Uryvayev. Topography and dynamics of synthesis of structural proteins of Newcastle disease virus. J. Bacteriol. 91:1902-1906. 1966.-Newcastle disease virus S and V antigens are synthesized in the cytoplasm, as revealed by the immunofluorescence method. In some experiments, S antigen was...

Little is known about Coxiella burnetii infection among cattle farmers and farm residents in China. Thus, the present study was conducted to detect the seroprevalence of C. burnetii infection and estimate associated risk factors among cattle farmers and farm residents in China. A cross-sectional study was designed, and sera of 362 people living or working on 106 cattle farms were tested for C. ...

To determine the seroprevalence of Borrelia burgdorferi in horses in Minnesota, the database of the Veterinary Diagnostic Laboratory, University of Minnesota, was searched over a ten year period (2001May 2010). A total of 1,260 equine serum samples submitted by 112 veterinary clinics were tested using an indirect fluorescent antibody test. Samples with titers of >1:320 were considered positive....

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respect...

The presented macroscopic FE-model allows to analyze the thermal fields and the resulting stress built up during Selective Laser Sintering. Process and material parameters are focused on Direct Metal Laser Sintering (DMLS). The FE-model is introduced and the assumptions for the model are given. Three different geometric models are discussed. The 3Dmodel shows the sintering of a single line, whe...

A single antigen indirect immunofluorescence test was used to screen for chlamydial antibody among Malaysian infants, children, sexually active adults and prostitutes. Of 794 serum samples tested, 361 (45.5%) were positive. Seropositivity increased with age and sexual activity and ranged from 10 to 16% among children under 10 years old to 94.4% among prostitutes. Pregnant women and female adole...

This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 ...

“SHE APPLICATION of the fluorescent antil)odiv technic for the identification of blood group antigens in crythrocvtcs offers a method of invcstigation of a variety of theoretical anci practical problems in imrnuunohematobogv and genetics. Iii 1958, studies from this laboratory were rcportcdim in which sj)ccific fluorescence of group A and group B crvthrocvtcs was achieved with htuuiiaii anti-A ...

The purpose of the present work was to identify Neospora caninum infections in beef bulls belonging to 19 herds from six counties located in the Corrientes province, Argentina. The presence of antibodies to N. caninum was evaluated in 305 serum samples of bulls (Bos taurus and Bos indicus). Age and breed were recorded. An indirect fluorescent antibody test was used to determine specific antibod...

Serum samples from 1077 dogs suspected of having Neospora caninum infections from 35 states in the United States and 3 provinces in Canada were tested for N. caninum IgG antibodies by the indirect fluorescent antibody test. Antibodies to N. caninum were found in 75 of 1077 (7%) of the samples. Twenty of the positive dogs were females, 17 were males and the sex was not recorded on 38 dogs. Chi s...