conventional cytogenetic is the standard technique for detection of philadelphia (ph) chromosome in chronic myeloid leukemia (cml). evaluation of abelson murine leukemia/breakpoint cluster region (abl/bcr) fusion using dual-colour fluorescence in situ hybridization (d-fish) is an alternative approach allowing rapid and reliable detection of the disease. we employed the technique of interphase d...

Karyotyping is a technique used to display and study the human chromosomes for detecting abnormalities, genetic disorders or defects. M-FISH (Multiplex Fluorescent In-Situ Hybridization) provides color karyotyping. In this paper, naïve Bayes classification of M-FISH chromosome images based on watershed based chromosome segmentation is presented. It is observed that the classification of the wat...

Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes speciÞ c for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly speciÞ c. Sensitivity testing showed that the probes were able to detect as low as 103 CFU/mL. In addition, FISH was carried out directly on positive blood culture...

Two-color fluorescent in situ hybridization (FISH) is a widely used technique for comparing relative gene expression patterns. Current two-color FISH protocols are not ideal for detecting weakly expressed transcripts or monitoring signal strength and background levels during the course of the reaction. Here we describe an improved FISH protocol using the conventional highly sensitive chromogeni...

objective: 22q11.2 chromosomal region is a hot spot for many cytogenetic rearrangements especially microdeletions which are responsible for digeorge and velocardiofacial syndromes. the most characteristic sign in these patients is congenital cardiac conotruncal anomalies. the gold standard diagnostic test for these microdeletions is fish (fluorescent in situ hybridization). however this diagnos...

Abstract Background To evaluate the clinical effectiveness of a novel CellDetect staining technique, compared with fluorescent in situ hybridization (FISH), and urine cytology, diagnosis urothelial carcinoma (UC). Methods A total 264 patients suspicious UC were enrolled this study. All tissue specimens collected by biopsy or surgery. Urine specimen was obtained for examinations prior to surgica...

Traditionally globe artichoke and leafy cardoon have been cultivated for use as vegetables but these crops are now finding multiple new roles in applications ranging from paper production to cheese preparation and biofuel use, with interest in their functional food potential. So far, their chromosome complements have been poorly investigated and a well-defined karyotype was not available. In th...

The construction of the 28-chromosome karyotype Dasypyrum breviaristatum was undertaken using multicolor non-denaturing fluorescent in situ hybridization (ND-FISH) and Oligo-FISH painting protocols. A novel wheat-D. line D2138 contained 44 chromosomes including a pair D. 6VbS.2VbL translocation chromosomes. Individual F2 F3 progenies cross between with wheat lines CM62, MY11 JM22, respectively,...

Fish oils have many dietary benefits, but due to their strong odors and rapid deterioration, their application in food formulations is limited. For these reasons, nano-liposome was used to nano-encapsulate fish oil in this study and encapsulated fish oil was utilized in fortifying yogurt. Physicochemical properties of produced yogurt including pH, acidity, syneresis, fatty acid composition, per...