Correct selection of coefficients for blasthole parameters in Ghalat limestone mine in Shahr-e-kord of Iran has resulted is designing and performing 20 blasting operations. After each blast, fragmented rocks larger than one square meter, called oversize, are estimated and prepared for secondary blasting. Next blasthole pattern were designed based on previous blasting results. The results of the...

We describe the sequences of six primer pairs for the PCR amplification of nuclear microsatellite markers in the tree shrews, Tupaia glis and T. belangeri. Multilocus genotyping based on non-destructive DNA sampling of live-trapped animals reveals high allelic variability (A) and heterozygosity (He) at these loci. Such characteristics make these genetic markers ideal for linkage mapping and com...

Numerous attempts have been made to control leptospirosis by using chemoprophylaxis, but with limited success. The present study was done investigate the antileptospiral potential of hexane, ethyl acetate and methanol extracts Zingiber zerumbet rhizomes. were assayed for activity broth microdilution method against Leptospira interrogans (serovar Batavie, Canicola, Australis) biflexa serovar Pat...

DNA fragmentation and the nuclear protein matrix in boar spermatozoa were simultaneously assessed using a specific variant of the sperm chromatin dispersion (SCD) test that allows direct visualization of DNA and nuclear proteins under standard conditions of chemical lysis. Nuclear proteins remaining after lysis were stained with the fluorochrome 2,7-dibrom-4-hydroxy-mercury-fluorescein for spec...

Sperm DNA fragmentation was analysed in 113 semen samples obtained from different rams over a period of one year. Semen samples were collected from: unvaccinated rams between January and June (Control group 1; CG1); vaccinated rams at least 70 days after vaccination between October and December (Control group 2; CG2); vaccinated rams 20 days after vaccination (Vaccinated group 1; VG1); and vacc...

The use of herbal remedies is very popular in the United States, with >80 million people buying plant-derived preparations that are often highly degraded or potentially contaminated with nonefficacious plant material. A method utilizing DNA-based markers to identify highly fragmented or powdered plant material sold as botanicals in dietary supplements has been developed. By incorporating and st...

A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping segments of HCMV DNA to substitute for the missing SV40 enhancer. Within HCMV, t...

Next Generation Sequencing requires the input of high molecular weight genomic DNA to construct quality libraries for whole genome bacterial sequencing. Large scale sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of the input DNA using high-throughput methods that are fast and cost effective. In this study, the Agilent 22...