We have generated transgenic potato plants (Solanum tuberosum) containing the potato phytochrome protein encoded by the PHYA gene cDNA (phyA) in sense or antisense orientation under the control of the 35S cauliflower mosaic virus promoter. Plants with increased and decreased phyA levels were analyzed. When grown under white light, development and growth of sprouts and plants were barely disting...

Potato virus Y (PVY) coat protein gene has been cloned from an Indian isolate of PVY. The coat protein gene from this isolate showed 93.2% nucleic acid sequence homology and more than 94% amino acid sequence homology compared to an American isolate. The coat protein gene has been sub-cloned into plant expression vector pBINPLUS and tobacco leaf discs were transformed using Agrobacterium tumefac...

this research was performed in order to study and diagnose potato virus y,potyvirus (pvy), potato virus x,potexvirus (pvx), potato virus s,carlavirus (pvs), potato virus a,potyvirus(pva) and potato leafroll luteovirus (plrv) on tubers and leaves of different potato varieties, namely, solea, safran, floris, proventa, milva, universa, lady olympia, vangogh, and marabel grown in afyon region of tu...

Like many plant RNA viruses, infection by potato spindle tuber viroid (PSTVd) is known to lead to RNA silencing and a marked reduction in visible disease. To examine the relationship between RNA silencing and this recovery phenomenon in greater detail, we have carried out time-course analyses of viroid-specific small RNA accumulation using several viroid—host combinations. These analyses reveal...

Verticillium wilt of potato is a major limiting factor in potato production caused by Verticillium dahliae. The objective of this study was to evaluate the efficacy two elicitors including Acibenzolar-S-methyl (ASM) and Chitosan as plant activator in controlling of potato verticillium wilt in vitro and greenhouse conditions. ASM and chitosan were tested in vitro using Potato dextrose Agar (PDA)...

Within a population of F hybrids between two genotypes ( L. Group Phureja DM 1-3 516 R44 [DM] and L. Group Tuberosum RH89-039-16 [RH]) used in the potato genome sequencing project, we observed fruit set after self-pollination on many plants. Examination of pollen tube growth in self-fertile and self-unfruitful F plants after controlled self-pollinations revealed no difference in the ability of ...

Various densities of adult potato leafhoppers were established on 'Essex' soybean at plant growth stage V2 in caged field plots. Cages were removed two weeks later, and plots remained uncaged for the rest of the growing season. Direct counts of nymphal leafhoppers on plants shortly after cage removal confirmed that a gradient of infestation levels was attained. In both years of the study, highe...

The presence of volunteer potato Solanum tuberosum L., cutleaf nightshade, S. triflorum N., and hairy nightshade, S. physalifolium Rusby (Solanales: Solanaceae), throughout potato crop rotations can diminish the effectiveness of crop rotations designed to control disease and pest problems associated with growing potatoes. In greenhouse bioassays, larvae of the Colorado potato beetle, Leptinotar...

ABSTRACT Potato seed is one of the largest costs a crop. This mostly due to incidence and spread viruses that occurs when crop grown in open field. Thus, it necessary obtain virus-free potato plantlets through meristem isolation, with subsequent cultivation protected environment, where easier control virus-spreading insects. One way increase greenhouse viability by multiplicating using high tec...