Exosomes are important vehicles of intercellular communication that shape host responses to physiologic, tumorigenic, and pathogenic conditions. The composition and function of exosomes are dynamic and depends on the state and condition of the cellular source. In prior work, we found that semen exosomes (SE) from healthy donors who do not use illicit drugs potently inhibit HIV-1. Following seme...

The relationship between FOS production in the sensory cortex and limbic system and the ability of C57BL/6N mice to acquire context- and tone-dependent freezing were investigated after fear conditioning, which was achieved by exposure of mice to context only or context and tone (10 kHz, 75 dB) as conditioned stimuli (Cs) paired with an electric footshock (0.7 mA, constant) as unconditioned stim...

With increasing concern about the ecological consequences of global climate change, there has been renewed interest in understanding the processes that determine species range limits. We tested a long-hypothesized trade-off between freezing tolerance and growth rate that is often used to explain species range limits. We grew 24 willow and poplar species (family Salicaceae) collected from across...

To investigate the load-bearing characteristics of anchor rods under asymmetric freezing conditions such as those in cold regions and effect frost swelling, a model test device was developed using controlled-temperature environmental box hydraulic actuator. Laboratorial pull-out tests soil layers were conducted at different moisture contents temperatures, changes rod force before after quantita...

Coagulated protein obtained by alkali extraction and acid precipitation from the bonecontaining residues discarded after mechanical separation of chicken was textured by freezing in semi-infinite cylinders followed by heat-setting in a microwave oven. Macrophotography was used to illustrate textural differences resulting from pH variations (4.5-6.0) in the precipitated protein, and changes in t...

Heat transfer during the freezing of guava pulp conditioned in large containers such as in stacked boxes (34 L) and buckets (20 L) and unstacked drums (200 L) is discussed. The air velocities across the crosssection of the tunnel were measured, and the values in the outlet of the evaporator were used as the initial conditions in computational fluid dynamics (CFD) simulations. The model tested w...

Heat capacities measured for isolated aluminum clusters show peaks due to melting. For some clusters with around 60 and 80 atoms there is a dip in the heat capacities at a slightly lower temperature than the peak. The dips have been attributed to structural transitions. Here we report studies where the clusters are annealed before the heat capacity is measured. The dips disappear for some clust...

Cells can endure storage at low temperatures such as--196 degrees C for centuries. The challenge is to determine how they can survive both the cooling to such temperatures and the subsequent return to physiological conditions. A major factor is whether they freeze intracellularly. They do so if cooling is too rapid, because with rapid cooling insufficient cell water is removed osmotically to el...

Low-temperature adaptation and cryoprotection were studied in the lactic acid bacterium Lactococcus lactis MG1363. An approximately 100-fold increased survival after freezing was observed when cells were shocked to 10 degrees C for 4 h compared to mid-exponential-phase cells grown at 30 degrees C, indicating an active protection against freezing. Using two-dimensional gel electrophoresis a grou...

Frozen soil increases overland flow and flood risk, but the question remains under which conditions frozen significantly impedes infiltration. In this study, we simulated infiltration into with a numerical model to investigate theoretical controls on for several types. We found that capacities of soils intermediate texture are most affected by freezing. Furthermore, examined influence initial s...