conclusions this study indicated that the optimization of gene transfer increases the expression of igf-1. high concentrations of igf-1, in combination with cell therapy, have a significant effect on wound healing. results in vitro, the maximum expression of igf1 (96.95 pg/ml) in transfected fibroblast cells was 24 hours after gene transfer. in vivo, it was clear that igf-1 gene therapy caused ...

Upon revisiting of our large library of archived human skin wound specimens, we observed that stratum lucidum interfollicular parakeratotic corneocytes appear to take an active role in epidermal wound healing by expanding, migrating and bifurcating to interact directly with and secure the wound scab in place. Our observations are based on the morphologic analysis of 240 acute, normal, full-thic...

OBJECTIVE To compare the healing of full-thickness skin punch wounds treated with topical autologous platelet gel (APG) vs conventional therapy (antibiotic ointment and/or occlusive dressings) in healthy volunteers. METHODS A prospective, single-blind, pilot study comprising 80 full-thickness skin punch wounds (4 mm diameter) was conducted on the thighs of 8 healthy volunteers. With each subj...

gum tragacanth is a natural complex mixture of polysaccharides and alkaline minerals extracted from species of astragalus plant, which is found widely in arid regions of the middle east. in a pilot experimental study we examined the effects of its topical application on wound healing in ten albino adult male rats. two similar parasagittal elliptical full-thickness wounds (control vs. test sampl...

exogenous nitric oxide donors such as deta nonoate, spontaneously release nitric oxide. this study aimed to investigate the effect of deta nonoate as a nitric oxide releasing drug on the rate of collagen synthesis during the impaired wound healing in a rat model of diabetes. twelve male sprague–dawley rats were transferred into separate metabolic cages. nine days before wounding, the rats were ...

The microorganisms have been usual noted as the major cause of delayed wound healing. Pseudomonas aeruginosa is the most common pathogen causing these infections. Silver nanoparticles (AgNPs) show ample antibacterial activities. In present study, the effect of AgNPs alone and in combination with tetracycline investigated on inoculated wounds with Pseudomonas aeruginosa in mice. Twenty mice anes...

Full-thickness wounds that have rendered patients candidates for amputation may require techniques that may include a combinatorial approach above traditional standard of care. The purpose of this retrospective study was to evaluate the effectiveness of an innovative approach whereby several therapies were combined to avoid amputation. Patients with full-thickness wounds who were previously rec...

This research was designed to study the effect of bitter almond oil on the healing of open skin wounds in rats. Thirty female adult rats were randomly divided into experimental (n=15) and control (n=15) groups. Under general anesthesia and sterile conditions, one quadrangular (surface area=22 mm) full thickness skin wound was made on the dorsum of each rat. The day of surgery was considered as ...

AIMS Stem cells are a new hope to ameliorate impaired diabetic wound healing. The purpose of this study was to evaluate the effect of adipose tissue derived mesenchymal stem cells (AD-MSCs) on wound healing in a diabetic rat model. METHODS Twenty-six rats became diabetic by a single intraperitoneal injection of streptozotocin. Six rats served as non-diabetic (non-DM). Diabetic rats were divid...

Objectives: The umbilicus is often used as a port insertion site during laparoscopic surgery because the wound invisible after surgery. However, its concave shape makes postoperative treatment difficult, resulting in umbilical keloids due to infection. Current study reports four cases of excision with satisfactory results following full-thickness skin grafts and radiation therapy.