BACKGROUND IgG and IgG subclass antibodies to Aspergillus fumigatus (A fumigatus) were measured in a large population of patients with cystic fibrosis to elucidate a putative antibody pattern specific for allergic bronchopulmonary aspergillosis (ABPA). METHODS An ELISA technique using water soluble somatic hyphal (WSSH) A fumigatus antigens and subclass specific monoclonal antibodies was used...

Detection of serum precipitins to Aspergillus fumigatus by counterimmunoelectrophoresis is compared with the immunodiffusion technique. Eight of nine (89%) sera from patients with proven A. fumigatus infection were positive by both methods. No serum from subjects with other systemic mycoses, bacterial infections, or healthy controls had detectable precipitins. The highest serum precipitin titer...

voriconazole is the first-line therapy for invasive aspergillosis. nevertheless, mortality remains high due to different factors. although azoles are very active in vitro against aspergillus fumigatus, several studies have reported azole-resistant a. fumigatus isolates in european countries, asia, and the united states. therefore, new approaches are needed. the aim of the present study was to e...

Introduction: Aspergillus fumigatus is the leading cause of invasive aspergillosis in immunocompromised patients with a high rate of mortality. Despite introduction of several classes of antifungal drugs, the limitations of current therapies have prompted an intense research toward the discovery of new antifungal compounds. In a recent study, several potential drug targets were identified based...

Roti tawar merupakan pangan yang banyak dikonsumsi manusia sebagai pengganti nasi. dapat bertahan tidak lebih dari seminggu sehingga mudah ditumbuhi oleh mikroorganisme seperti jamur. kadaluarsa ini didapatkan salah satu toko di Daerah Antang Kota Makassar. Jamur biasanya mengkontaminasi roti adalah Aspergillus sp yaitu jamur mampu menghasilkan aflatoksin berbahaya bagi kesehatan manusia. Tujua...

The objective of the present study was to isolate fungi from agricultural soils and evaluate fungal growth in culture medium contaminated with atrazine, glyphosate pendimethalin. Filamentous were isolated cultured a modified containing 0, 10, 20, 50, 100 ?g mL-1 pendimethalin for 14 days at 28°C. that presented optimal satisfactory plated Sabouraud 4% dextrose herbicides concentrations seven me...

the disposed wastes of zinc industries contain large amounts of heavy metals such as pb2+ and cd2+. these elements are considered as hazardous elements to human beings and other organisms. this study aims at introducing the fungal isolates that evolved to be compatible with waste deposits during the time and can absorb pb2+ and cd2+ions. during the spring and autumn in 2011, eight samples of se...

Two erg3 genes encoding C-5 sterol desaturase enzymes (Erg3A and Erg3B) in Aspergillus fumigatus were characterized with respect to their nucleotide sequences and null mutant phenotypes. Targeted disruption of the erg3A and erg3B genes and a double gene knockout, erg3A- erg3B-, showed that they are not essential for A. fumigatus viability. Mutant phenotypes clearly showed that Erg3B is a C-5 st...

A panel of cDNAs encoding allergenic proteins was isolated from an Aspergillus fumigatus cDNA library displayed on the surface of filamentous phage. Solid phase-immobilized serum immunoglobulin E (IgE) from A. fumigatus-allergic individuals was used to enrich phage displaying IgE-binding molecules. One of the cDNAs encoded a 11.1-kD protein that was identified as acidic ribosomal phosphoprotein...

For industrial applications in animal feed, a phytase of interest must be optimally active in the pH range prevalent in the digestive tract. Therefore, the present investigation describes approaches to rationally engineer the pH activity profiles of Aspergillus fumigatus and consensus phytases. Decreasing the negative surface charge of the A. fumigatus Q27L phytase mutant by glycinamidylation o...