Overexpression of the transcription factor Spi-1/PU.1 in mice leads to acute erythroleukemia characterized by a differentiation block at the proerythroblastic stage. In this study, we made use of a new cellular system allowing us to reach graded expression of Spi-1 in preleukemic cells to dissect mechanisms of Spi-1/ PU-1 in erythroleukemogenesis. This system is based on conditional production ...

Previously it was shown that the Ets proteins, PU.1 and Spi-B, exhibit functional redundancy in B lymphocytes. To investigate the possibility that PU.1 or Spi-B or both share overlapping roles with Ets-1 or Elf-1, PU.1(+/-)Ets-1(-/-), PU.1(+/-)Elf-1(-/-), and Spi-B(-/-)Ets-1(-/-) animals were generated. No blood cell defects were observed in these animals except those previously reported for Et...

Acute leukemias are characterized by deregulation of transcriptional networks that control the lineage specificity of gene expression. The aberrant overexpression of the Spi-1/PU.1 transcription factor leads to erythroleukemia. To determine how Spi-1 mechanistically influences the transcriptional program, we combined a ChIP-seq analysis with transcriptional profiling in cells from an erythroleu...

In April 2007, FUJIFILM introduced ETERNA-RDI for digital intermediate. It is the first film stock in the world, designed specially for use in the digital intermediate workflow. ETERNA-RDI offers a significant improvement over conventional non-specific intermediate film stock, producing fine detail and accurate color from digital image data. New silver halide grain technology prevents spectral ...

The ETS protein Spi-1/Pu.1 plays a pivotal and widespread role throughout hematopoiesis in many species. This study describes the identification, characterization, and functional analysis of a new zebrafish spi transcription factor spi-1– like (spi-1l) that is expressed in primitive myeloid cells, erythro-myelo progenitor cells, and in the adult kidney. Spi-1l functions genetically downstream o...

Appears in: EDULEARN22 Proceedings Publication year: 2022Pages: 4029-4037ISBN: 978-84-09-42484-9ISSN: 2340-1117doi: 10.21125/edulearn.2022.0968Conference name: 14th International Conference on Education and New Learning TechnologiesDates: 4-6 July, 2022Location: Palma, Spain

In order to define a modern approach for Software Process Improvement (SPI), the SPI Manifesto was developed, discussed, and finalized at the 16 EuroSPI Conference in 2009 in Alcala, Spain. The common understanding gained during the discussion and usage of the manifesto formed a nucleus from which the new ISO/IEC 33014[ISO01] was derived. In parallel to the development of the SPI Manifesto, the...

To optimize irrigation, agronomists need to modulate crop water productivity (CWP) throughout phenology. We compared regulated deficit irrigation (RDI) and sustained (SDI) in chickpea (Cicer arietinum L. var. Blanoro), expecting RDI during vegetative growth (VG) enhance CWP, as opposed flowering (F) pod-filling (PF) stages. The effects of SDI on grain yield, plant height, weight, caliber, pods ...

The Spanish market offers a significant number of folic acid (FA) voluntarily fortified foods. We analysed FA and (6S)-5-methyltetrahydrofolic acid ((6S)-5-CH3-H4PteGlu) content in ready-to-eat cereals (RTEC) (n=68) and cow's milk (n=25) by a previously validated affinity chromatography-HPLC method. Contribution to potential FA intakes for children aged 2-13years, was assessed using food consum...