Two α-galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28°C were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65°C, respectively. The KM app for ρ-nitrophenyl-α-D-galactopyranos...

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The production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way a...

Probiotics improve the intestinal microbiota and exert benefi ts for the host (Fuller, 1989; Parvez et al., 2006). Some strains of lactobacilli utilize lactose and alleviate lactose intolerance (Montalto et al., 2006). Lactobacillus gasseri is one of the predominant lactobacilli in the human intestine, and strains are used in probiotic foods. Lactobacillus gasseri is the major homofermentative ...

Plant parasitic nematodes need to overcome the barrier presented by plant cell wall in order invade their host. A variety of degrading enzymes are present endoparasitic including that degrade cellulose (beta 1,4 endoglucanases) and various pectin components. We describe cloning functional analysis genes encoding GH53 arabinogalactan endo-1,4-beta-galactosidases from three related Globodera rost...

Thermostable β-galactosidases from thermophilic bacteria have attracted increasing interest to various advantages in industrial and biotechnological applications. In this study, a highly thermally stable β-galactosidase produced by Anoxybacillus sp. AH1was purified characterized. The highest enzyme production was achieved after the bacterium incubated for 24 hours. precipitation with ammonium s...