BACKGROUND/PURPOSE To investigate the in vitro and in vivo activity of imipenem-colistin combination against multidrug-resistant Enterobacter cloacae infections in order to determine whether it should be explored further. METHODS The antimicrobial activity of colistin alone and in combination with imipenem was assessed versus an imipenem-susceptible isolate, E. cloacae GN1059, or an imipenem-...

We report the isolation of a bacterium from Galleria mellonella larva and its identification using genome sequencing and phylogenomic analysis. This bacterium was named Alcaligenes faecalis strain MOR02. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora. Using G. mellonella larvae...

Thermolysin-like metalloproteinases such as aureolysin, pseudolysin, and bacillolysin represent virulence factors of diverse bacterial pathogens. Recently, we discovered that injection of thermolysin into larvae of the greater wax moth, Galleria mellonella, mediated strong immune responses. Thermolysin-mediated proteolysis of hemolymph proteins yielded a variety of small-sized (<3 kDa) protein ...

We analyzed the antifungal susceptibility profiles, genotypes, and virulence of clinical Aspergillus terreus isolates from six university hospitals in South Korea. Thirty one isolates of A. terreus, comprising 15 respiratory and 16 ear isolates were assessed. Microsatellite genotyping was performed, and genetic similarity was assessed by calculating the Jaccard index. Virulence was evaluated by...

Immune responses play a key role in the survival of all living things. Intake inorganic materials is critical importance organisms because their participation metabolism and some component cells. Iron (Fe), magnesium (Mg) selenium (Se) were selected for this study constituents cells, such as nucleic acids enzymes organisms, indirect direct effects on immunity. Although separate these are known,...

There is growing pressure to find a way eradicate or reduce the levels of foodborne pathogens such as Campylobacter in broiler chickens, whilst limiting use antimicrobials. For Campylobacter, there currently no vaccine and on-farm biosecurity alone insufficient prevent colonization chicken flocks. Dipteran flies are proven carriers their entry into houses may contribute its transmission chicken...

Adhesins facilitate bacterial colonization and invasion of host tissues are considered virulence factors, but their impact on immune-mediated damage as a driver pathogenesis remains unclear. Yersinia pseudotuberculosis encodes for multivalent adhesion molecule (MAM), mammalian cell entry (MCE) family protein adhesin. MAMs widespread in Gram-negative bacteria enable enteric to colonize epithelia...