We evaluated simultaneous analytical methods for organophosphorus pesticides in cruciferous vegetables by gas chromatography (GC). Firstly, 36 pesticides were selected on the basis of monitoring data (April 1996-March 2003) in Aichi Prefecture. A sample was extracted with acetonitrile and the acetonitrile layer was separated by salting-out. The extract was cleaned up with gel permeation chromat...

Aromatic L-amino acid decarboxylase was purified from bovine brain for the first time by affinity chromatography using a monoclonal antibody to the enzyme, and it was compared with the decarboxylase purified from bovine adrenal medulla by the same procedure. The monoclonal antibody was produced from a hybridoma established for the enzyme highly purified from bovine adrenal medulla. The Mr value...

Liquid chromatography has been used as a means of egg protein analysis or as a method for the purification of egg proteins. Several chromatographic methods, including gel permeation, ion-exchange, reversed-phase, hydrophobic interaction, and immobilized-ligand-affinity chromatography, have been carried out for the separation or the purification of egg yolk or egg white proteins. Ion-exchange ch...

The methanolic extract from the barks of the medicinal plant Qualea parviflora (Vochysiaceae) was fractionated by column chromatography over silica gel followed by gel permeation over Sephadex LH-20 to give 3,3'-di-O-methylellagic acid-4-O-beta-D-glucopyranoside (1), 3-O-methylellagic acid-4'-O-alpha-L-rhamnopyranoside (2), 3,3',4-tri-O-methylellagic acid-4'-O-beta-D-glucopyranoside (3), and 3,...

General. 1H and C NMR spectra were measured on Varian Gemini 2000 (300 MHz for H) instruments, and are reported in parts per million (δ) from internal tetramethylsilane or residual solvent peak. IR spectra were recorded on a Shimadzu FTIR-8200, and absorptions are reported in cm. FAB-MS spectra were recorded on JEOL IMS-300 spectrometer. 3-Nitrobenzyl alcohol was used as a matrix for FAB-MS. Me...

The controlled synthesis of terpolymer structures is often limited by the intrinsic reactivities monomers. For a statistical from cyclohexene oxide (CHO) and propylene (PO) with CO2, an instrumental solution demanded. Implementing setup where one monomer can be added to reaction mixture over whole course reaction, random distribution epoxides chain realized. successful terpolymerization determi...