This study aims at discussing several methods to produce aluminum oxide (Al2O3) synthesis along with the advantages and disadvantages of each method used. In general, are available: (1) precipitation, (2) combustion, (3) sol-gel, (4) wet chemical, (5) in supercritical water conditions, (6) microwave, (7) mechanochemical, (8) hydrolysis, most efficient for synthesizing Al2O3 is precipitation bec...

Objective: The aim of this study was to obtain protein isolate from sacha inchi using alkaline pH at different pHs of precipitation with water and salt and to analyze protein isolate with electrophoresis. Methods: Sacha inchi protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using electrophoresis native-polyacrylamide gel electrophore...

A lytic enzyme induced in Micrococcus lysodeikticus strain 1 by infection with N1 bacteriophage was purified 45- to 50-fold by ammonium sulfate precipitation, acid precipitation, and selective adsorption of contaminating proteins with calcium phosphate gel. The optimal pH for activity of the enzyme was 6.5 to 7.0. Maximal activity occurred at 45 to 50 C and at an ionic strength of 0.06. The enz...

Hummel’s (1) fluorometric determination of malic acid requires the precipitation of calcium malate by alcohol as a means of fractionation from biological materials. Fructose-l, 6-diphosphate, glucose-6-phosphate, or glycogen if present must be removed. Below is described a method for preparing extracts of tissues which applies the principles of chromatography on silica gel. Osazone formation, p...

The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation...


 Nickel ferrite (NiFe2O4) is one of the spinel nanoparticles that useful for scientific and technological, like biomedicine. This paper discusses synthesis NiFe2O4 by various methods. aims to find out most efficient method which are used as hyperthermia agents in The a literature study an article with publications from 1956-2018 discussing several methods syntheses, such sol-gel, hydrothe...

Further evidence of a cross-reactive relationship between a cell wall antigen of certain Group A streptococcal strains and mammalian heart tissue is provided by the demonstration that goat antisera to washed human or rabbit heart tissue homogenates exhibit precipitation with cell wall extract preparations. A single line of precipitation was observed in agar gel diffusion tests. Precipitating ac...

Transglutaminase (TGase) is widely used in the food industry. In this study, TGase was purified by affinity precipitation using l-thyroxin, coupled to a thermo-responsive polymer (PNBN), as an affinity ligand. The lower critical solution temperature and recovery of the affinity polymer were 31.0 °C and 99.6 %, respectively. The optimal adsorption condition was 0.02 mol/L phosphate buffer (pH 5....

A strong measles-specific gel precipitation reaction was found in the cerebrospinal fluid (C.S.F.) of two patients with multiple sclerosis (M.S.) (total of 15 tested). The serum and C.S.F. specimens from these two patients were tested for measles antibody by six assay methods. The results were compared with those obtained from serum and C.S.F. specimens of a patient with subacute sclerosing pan...

The aim of this study was to characterize of chicken egg yolk immunoglobulins (IgYs) specific as immunotherapy to Mycobacterium tuberculosis complex (MTBC) infection. Lohmann laying hens were immunized intramuscularly with antigenic of MTBC. Egg yolk was separated from egg white, and IgY antibody was then purified by multiple polyethylene glycols 6000 extraction and ammonium sulfate purificatio...