نتایج جستجو برای: genomic dna
تعداد نتایج: 582362 فیلتر نتایج به سال:
Many DNA base substitution models have been proposed to describe the evolution of short DNA sequences. Most of these models are special reversible time-continuous Markov chains on the DNA bases. A general reversible model for long genomic DNA sequences, which includes the previous models as special cases, is described in this paper. To estimate the model parameters from pairwise alignments, two...
background chronic hepatitis b virus (hbv) infection is a multifactorial disease that can result in serious clinical complications. host genetic background especially the genes that encode immunologic factors like inf-γ and its receptor (ifn-γ r) are critical in the pathogenesis of infection. materials and methods genomic dna from peripheral blood samples of 200 chronically hbv infected patient...
genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...
Background: Existence of bacterial host cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor, intensive and rather exp...
BACKGROUND The PacBio RS II provides for single molecule, real-time DNA technology to sequence genomes and detect DNA modifications. The starting point for high-quality sequence production is high molecular weight genomic DNA. To automate the library preparation process, there must be high-throughput methods in place to assess the genomic DNA, to ensure the size and amounts of the sheared DNA f...
We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
use of random amplified polymorphic dna to identify candida species, originated from cancer patients
introduction: recently, the incidence of fungal infections dramatically increased with an appearance of many novel species due to different criteria. therefore, several molecular methods have been established for identification of these agents which cause human and animal disorder based on genomic dna. among these methods, rapd-pcr technique is a powerful discriminative method based on amplifie...
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