Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

abstract genomic dna extraction with high quantity and quality is the basic necessities of molecular genetic studies. the aim of this study is the applicability of different dna extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snail conus coronatus, mollusca with phenol-chloroform, ammonium ac...

The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye K...

this research was carried out for evaluation of competitive ability of some wheat cultivars (triticum aestivum l.) of recommended wheat in dargaz region with common weed of feral rye (secale cereale). experiment was conducted as factorial based on randomized complete block design with three replications in 2010-2011 growing season. experimental factors were included four wheat cultivars of says...

introduction: respiratory pathogen, chlamydia pneumoniae, has been implicated in the pathogenesis of coronary artery disease. previous studies have demonstrated an antichlamydial seroresponse to be a cardiovascular risk factor and coronary atheromata to contain chlamyial components in varing proportions. endovascular demonstration of bacterial dna is required to provide evidence for an infectio...

in recent years, rye caused heavy damages in wheat farms in iran. in order to evaluate wheat competitiveness ability against rye, an experiment was conducted in replacement series design. field experiment was carried out at the agricultural and natural resources research station in miandoab during 2009-2010 (for 2 years). the experimental design was a randomized complete block design with 3 rep...

BACKGROUND Epidemiological studies show inverse relationship between intake of wholegrain cereals and several chronic diseases. Components and mechanisms behind possible protective effects of wholegrain cereals are poorly understood. OBJECTIVE To characterise commercial rye bran preparations, compared to wheat bran, regarding structure and content of nutrients as well as a number of presumabl...

A linear UV dose response is demonstrated using an ELISA assay that measures thymine dimers formed in the genomic DNA extracted from mixed species of microorganisms filtered treated pre-disinfection drinking water.

Some environmental mutagenic agents cause genomic instability and increase susceptibility of DNA damage. One of them is mitomycin C which is connected to DNA as an alkylating factor and affects susceptible cells to reduction reactions. This drug is used in chemotherapy and treatment of tumors. Study of genomic instability in the presence of different concentrations of MMC can show susceptibilit...