Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and tryp...

Stavudine (Zerit, d4T) is widely used as an anti HIV infection drug. It prevents HIV by altering the genetic material of healthy cells but causes mutations in mitochondrial and nuclear DNA. It also produces clastogenic effects in mice. In the present investigation, comet assay test was applied to evaluate the possible genomic damage caused by stavudine and also the ameliorating effects of garli...

Harmful effects of contaminants on the ecosystem and humans can not be assessed by standard chemical analyses of environmental samples, therefore toxicity tests using live organisms or cells represent a vital part of environmental monitoring. Many different biological methods based on native or genetically modified microorganisms as test-species, have already successfully been applied to enviro...

In this study, subcritical water was used for the extraction and fractionation of organic atmospheric particulate. Two types of carbonaceous aerosol, diesel exhaust particulate (a relatively nonpolar matrix) and wood smoke particulate (a polar matrix), were sequentially extracted using subcritical water at temperatures ranging from 25°C to 300°C. The importance of each fraction was evaluated us...

The micronucleus (MN) test in bone-marrow and peripheral blood erythrocytes is one of the best established in vivo cytogenetic assays in the field of genetic toxicology, providing a convenient and reliable index of both chromosome breakage and chromosome loss. Therefore a study was made on micronuclei assay to evaluate the degree of genotoxicity of acrylamide and its toxicologic effects on chic...

Introduction Direct pulp capping (DPC) is a treatment for maintaining pulp vitality and its biological function. Ideally, pulp capping agents are expected to induce pulp cells to form hard tissue. This in vitro study assessed the cytotoxicity and genotoxicity of three vital pulp capping (VPC) agents naming Biodentine (Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (ProRoo...

The ecological and health concern of mutagenicity and carcinogenicity potentially associated with an overwhelmingly large and ever-increasing number of chemicals demands for cost-effective and feasible method for genotoxicity screening and risk assessment. This study proposed a genotoxicity assay using GFP-tagged yeast reporter strains, covering 38 selected protein biomarkers indicative of all ...

This technical report describes an approach developed by the EFSA cross-cutting Working Group on Genotoxicity for reporting of reliability and relevance genotoxicity studies. The scope this document is to ensure harmonisation transparency evaluation evidence among Units dealing with scientific assessments. It recommended be used as a template drafting assessments in Opinions.