Rapid diagnostic tests (RDTs) capable of detecting and differentiating Plasmodium species are needed in areas in which microscopy is unsuitable. This study was conducted to assess the diagnostic performance of the rapid test kit - SD BIOLINE Malaria Ag Pf/Pan(r) (05FK60) in an endemic area. Microscopy of Giemsa-stained blood films were performed to detect and estimate the Plasmodium density in ...

Chromosomes from successive passages of a Chinese ham ster cell strain (WCHE/5) that spontaneously progressed from a euploid primary cell culture to a heteroploid tumorigenic cell line were isolated and analyzed by Giemsa banding and high-reso lution flow karyotype analysis. The frequency and identification of aneuploid and marker chromosomes were determined at both preand postcrisis culture st...

Human metaphase chromosomes were photooxidized in the presence of methylene blue, a process that destroys guanine residues in DNA. Indirect immunofluorescence showed that such chromosomes reacted with a cytosine-specific antibody revealing a consistent fluorescent banding pattern by which each chromosome could be identified. The observed fluorescent patterns were the reverse of those produced i...

The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chr...

The prevalence of cryptosporidial infection among captive wild animals and birds in Sanda Kyarimi Park, Maiduguri, north-eastern Nigeria, was evaluated by faecal examination for oocysts using three different staining techniques: the modified Ziehl-Neelsen, Giemsa, and Safranin-methylene blue. A total of 66 captive wild animals and birds were examined based on age and sex differences. Fifteen (2...

Cytogenetic studies from case 1 included cultures of peripheral blood and skin fibroblasts. Karyotypes from both cultures showed trisomy D(47,XX, + D). Giemsa banding of cells from the skin fibroblast culture, using a modification of the method of Seabright (1971), showed 47,XX,+ 13 (Fig. 3). Chromosome analysis of peripheral leucocytes of the parents were normal. In case 2 the karyotype from a...